Fabrication of Calcium Sulfate Coated Selenium Nanoparticles andCorresponding In-Vitro Cytotoxicity Effects Against 4T1 Breast Cancer Cell Line

Background: the inhibitory effect of selenium nanoparticles (senps) on cancer cellshas been reported in many studies. in this study, the purpose was to compare the invitro effects of senps and calcium sulfate coated selenium nanoparticles (caso4@senps) on breast cancer cells.methods: caso4@senps and senps were chemically synthesized and characterizedwith field emission scanning electron microscope (fesem) and energy-dispersive xrayspectroscopy (edx). by applying mtt assay, the cytotoxicity effect of both nanomaterialson the 4t1 cancer cells was investigated.results: while ld50 of senps on 4t1 cancer cells was 80 μg, the ld50 of caso4@senpswas reported to be only 15 μg. the difference between the inhibition rates obtainedfor senps and caso4@senps was statistically significant (p=0.05). in addition, athigher concentrations (50 μg) of caso4@senps, the cytotoxicity was 100% more thansenps alone.conclusion: according to the result of the present work, it can be concluded that decorationof senps with calcium sulfate leads to an increase in potency by decreasingthe effective dose. this effect can be attributed to activation of intrinsic apoptosissignaling and/or ph regulatory properties of caso4@senps. however, further studiesare still needed to determine the exact corresponding mechanisms of this synergisticeffect.