The Effect of Silver Nanoparticles on Pyocyanin Production of Pseudomonasaeruginosa Isolated From Clinical Specimens

Background: pseudomonas aeruginosa (p. aeruginosa) is an opportunistic pathogencausing a wide range of human infections. the organism is resistant to a wide rangeof antibiotics. the purpose of this study was to investigate the effect of agnps on pyocyaninpigment production of p. aeruginosa bacteria isolated from clinical specimens.methods: in this study, 15 clinical isolates of p. aeruginosa were collected from differentspecimens of hospitalized patients. p. aeruginosa was detected by biochemical andmolecular (detection of pbo1 gene by colony pcr method) methods and the mic andmbc of agnps were determined by agar dilution method. inhibition of p. aeruginosapyocyanin production at agnps concentrations of 0, 0.3, 0.5, 1 and 1.5 mg/ml of wasstudied with od of 520 nm.results: the mean mic and mbc of agnps were 1.229 and 1.687 mg/ml, respectively.pyocyanin production was investigated for all isolates at different concentrations ofnanoparticles, and their comparison showed that with increasing nanoparticle concentration,pyocyanin production significantly decreased (p<0.05).conclusion: according to the results of this study, agnps had an inhibitory effect on p.aeruginosa and its pigment production and with increasing nanoparticles concentration,pigment production decreased; therefore, it seems that the nanoparticles can beused to treat and prevent diseases caused by p. aeruginosa.