β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human ColonCancer HT-29 Cells

Background: silver nanoparticles (agnp) are commonly used metallic nanoparticlesin health care systems. colon cancer incidence is increasing worldwide. in this study,agnp was synthesized using β-sitosterol and its cytotoxic potential was evaluated inhuman colon cancer (ht-29) cells.methods: characterization of agnp was analyzed by tem and spectrophotometryanalysis. ht-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/ml) of agnps and cytotoxicity was evaluated by mtt assay. the apoptosis was analyzedby the flow cytometry. the expression of p53 protein was analyzed by westernblotting.results: β-sitosterol mediated agnp are spherical in shape and induced concentration-dependent cytotoxicity in ht-29 cells. agnp caused apoptosis related morphologicalchanges as evidenced by annexin positive staining. agnp treatments also inducedthe p53 expression in ht-29 cells.conclusion: our present result suggests that β-sitosterol mediated agnp induce apoptosisin colon cancer cells and this finding may pave the way for further experimentalanalysis in vivo.