|
|
|
|
Production of Monoclonal Antibody against Human Nestin
|
|
|
|
|
|
|
|
نویسنده
|
Hadavi Reza ,Zarnani Amir Hassan ,Ahmadvand Negah ,Mahmoudi Ahmad Reza ,Bayat Ali Ahmad ,Mahmoudian Jafar ,Sadeghi Mohammad-Reza ,Soltanghoraee Haleh ,Akhondi Mohammad Mehdi ,Tarahomi Majid ,Jeddi-Tehrani Mahmood ,Rabbani Hodjattallah
|
|
منبع
|
avicenna journal of medical biotechnology - 2010 - دوره : 2 - شماره : 2 - صفحه:69 -77
|
|
چکیده
|
We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any n- or o-glycosylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. a wide variety of nestin proteins ranging from 140-250 kda was detected by this antibody. this antibody is highly specific and functional in applications such as elisa, flow cytometry, immunocytochemistry, and western blot assays.
|
|
کلیدواژه
|
Antibodies ,Blotting ,Immunohistochemistry ,Monoclonal ,Nestin ,Peptides ,Western
|
|
آدرس
|
Avicenna Research Institute, Monoclonal Antibody Research Center, ایران, Avicenna Research Institute, Nanobiotechnology Research Center, ایران, Avicenna Research Institute, Monoclonal Antibody Research Center, ایران, Avicenna Research Institute, Monoclonal Antibody Research Center, ایران, Avicenna Research Institute, Monoclonal Antibody Research Center, ایران, Avicenna Research Institute, Monoclonal Antibody Research Center, ایران, Avicenna Research Institute, Reproductive Biotechnology Research Center, ایران, Avicenna Research Institute, Reproductive Biotechnology Research Center, ایران, Avicenna Research Institute, Reproductive Biotechnology Research Center, ایران, Avicenna Research Institute, Reproductive Biotechnology Research Center, ایران, Avicenna Research Institute, Monoclonal Antibody Research Center, ایران, Avicenna Research Institute, Monoclonal Antibody Research Center, ایران
|
|
پست الکترونیکی
|
hodrab@ki.se
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|