Construction of A High Efficiency Pcr Products Cloning T Vector Using Pgem-5zf (+)

A highly efficient cloning vector was constructed for cloning pcr products by inserting an 80 bp dna fragment into pgem-5zf (+) vector. the xcm i digestion of this vector gave rise to a 3’ overhanging deoxythymidine offering the possibility of cloning pcr products with 3' adenosine overhang created by taq dna polymerase. furthermore, two ecor i sites were added to the construct for identification of recombinant plasmids using a single restriction enzyme. taken together, the more efficient cloning performance and the lower cost of this vector as compared to the commercial t vector, suggests that it may be one of the best t vectors for cloning of pcr products.