statistical optimization of process parameters by central composite design (ccd) for anenhanced production of l-asparaginase by myroides gitamensis bsh-3, a novel species

Background: the present study focused on the production of l-asparaginase using solid state fermentation (ssf) by myroides gitamensis. methods: initially, five significant parameters (carbon source; nitrogen source, temperature, ph and incubation period) were identified that affect the production process of l-asparaginase using classical one factor at a time (ofat) optimization. an optimized l-asparaginase specific activity obtained by ofat was recorded as 85.7 iu. central composite design (ccd) was also employed successively to optimize the multiple parameters at a time and their results were compared. results: maximum l-asparaginase enzyme specific activity obtained by ccd method was 295.6 iu under the hold values of carbon source (wheat bran) 12 g/l, nitrogen source (yeast extract) 7 g/l, temperature 37oc, ph=7.5 and incubation period 47 hr. upon validation, the obtained results proved that there was a good relation existing between the experimental and the predicted model (p<0.05). l-asparaginase activity was enhanced in statistical method up to 3.4 folds compared to that of classical method. conclusion: utilization of wheat bran as a low cost carbon source in ssf for the production of l-asparaginase enzyme makes the process economical and in turn reduces the environmental pollution by biotransformation to commercially useful bio product.