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Cloning and Expression of B. Mellitensis Bp26 Gene in Lactococcus Lactis As A Food Grade Vaccine
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نویسنده
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Azizpour Maghvan Maryam ,Jafari Parvaneh ,Hosseini Davood ,Behrozikhah Ali Mohammad
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منبع
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Avicenna Journal Of Medical Biotechnology - 2019 - دوره : 11 - شماره : 3 - صفحه:264 -267
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چکیده
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Background: brucellosis is still an important health problem in under developing countries and researches for finding efficient vaccine are going on. brucella melitensis (b. mellitensis) bp26 gene is a good candidate for brucellosis vaccine and investigations showed that lactococcus lactis (l. lactis) with several positive characteristic are attractive for protein expression as a live delivery vectors. these fast growing bacteria need no aeration, are easy to handle, have no exotoxin, endotoxin and protease, so the cost of culturing is inexpensive. methods: b. mellitensis bp26 gene was cloned in food grade pnz 8149 vector and expressed in l. lactis nz 3900. results: results showed that we can produce a food-grade recombinant l. lactis producing the b. melitensis bp26 protein. conclusion: in this study, for future evaluation about ability of l. lactis as a live delivery vector, a food-grade recombinant l. lactis producing the b. melitensis bp26 protein was produced.
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کلیدواژه
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Brucellosis ,Exotoxins ,Lactococcus Lactis ,Vaccines
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آدرس
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Islamic Azad University, Arak Branch, Department Of Microbiology, Iran, Islamic Azad University, Arak Branch, Department Of Microbiology, Iran, Agricultural Research, Education & Extension Organization, Razi Vaccine And Serum Research Institute, Iran, Agricultural Research, Education & Extension Organization, Razi Vaccine And Serum Research Institute, Iran
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Authors
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