cloning and expression of b. mellitensis bp26 gene in lactococcus lactis as a food grade vaccine

Background: brucellosis is still an important health problem in under developing countries and researches for finding efficient vaccine are going on. brucella melitensis (b. mellitensis) bp26 gene is a good candidate for brucellosis vaccine and investigations showed that lactococcus lactis (l. lactis) with several positive characteristic are attractive for protein expression as a live delivery vectors. these fast growing bacteria need no aeration, are easy to handle, have no exotoxin, endotoxin and protease, so the cost of culturing is inexpensive. methods: b. mellitensis bp26 gene was cloned in food grade pnz 8149 vector and expressed in l. lactis nz 3900. results: results showed that we can produce a food-grade recombinant l. lactis producing the b. melitensis bp26 protein. conclusion: in this study, for future evaluation about ability of l. lactis as a live delivery vector, a food-grade recombinant l. lactis producing the b. melitensis bp26 protein was produced.