ﺑﯿﺎن اﻓﺘﺮاﻗﯽ ژنﻫﺎی stftsh6 ،stftsh5 و stftsh10 در ﺳﯿﺐزﻣﯿﻨﯽ در ﻣﻮاﺟﻬﻪ ﺑﺎ ﺗﻨﺶﻫﺎی ﺳﺮﻣﺎ، ﮔﺮﻣﺎ و ﻧﻮر زﯾﺎد

ﻣﻘﺪﻣﻪ و ﻫﺪف: ﺗﻨﺶﻫﺎی ﻏﯿﺮزﯾﺴﺘﯽ ﻓﺎﮐﺘﻮرﻫﺎی ﻣﻬﻢ در ﮐﺎﻫﺶ ﻋﻤﻠﮑﺮد ﻣﺤﺼﻮﻻت زراﻋﯽ ﻫﺴﺘﻨﺪ. ﺳﺮﻣﺎ، ﮔﺮﻣﺎ و ﻧﻮر زﯾﺎد ازﺟﻤﻠﻪ ﻣﻬﻤﺘﺮﯾﻦ ﺗﻨﺶﻫﺎی ﻏﯿﺮزﯾﺴﺘﯽ در ﮔﯿﺎﻫﺎن ﺑﻪﺷﻤﺎر ﻣﯽروﻧﺪ. ﻟﺬا درک ﻣﮑﺎﻧﯿﺴﻢ ﻣﻮﻟﮑﻮﻟﯽ ژنﻫﺎی درﮔﯿﺮ در ﺗﻨﺶ ﺿﺮوری ﺑﻪﻧﻈﺮ ﻣﯽرﺳﺪ. ﻣﺘﺎﻟﻮﭘﺮوﺗﺌﺎز ftsh ﯾﮑﯽ از ﺗﻨﻈﯿﻢﮐﻨﻨﺪهﻫﺎی ﮐﻠﯿﺪی در ﭘﺎﺳﺦ ﺑﻪ ﺗﻨﺶﻫﺎی ﻏﯿﺮزﯾﺴﺘﯽ اﺳﺖ. ﻣﻄﺎﻟﻌﺎت زﯾﺎدی ﺑﺮ روی ftsh در ﮔﯿﺎﻫﺎن ﻣﺨﺘﻠﻒ اﻧﺠﺎم ﺷﺪه اﺳﺖ. اﻣﺎ ﺗﺤﺖ ﺗﻨﺶ ﺳﺮﻣﺎ، ﮔﺮﻣﺎ و ﻧﻮر زﯾﺎد ﺑﺮ روی ﺳﯿﺐزﻣﯿﻨﯽ ﮔﺰارﺷﯽ وﺟﻮد ﻧﺪارد.ﻣﻮاد و روشﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، ﺑﯿﺎن ژن stftsh6 ،stftsh5 و stftsh10 در ﺳﻪ ﺑﺎﻓﺖ رﯾﺸﻪ، ﺳﺎﻗﻪ و ﺑﺮگ در ﭘﺎﺳﺦ ﺑﻪ ﺳﻪ ﺗﻨﺶ ﺳﺮﻣﺎ، ﮔﺮﻣﺎ و ﻧﻮر زﯾﺎد ﺑﺮرﺳﯽ ﺷﺪ. ژﻧﻮﺗﯿﭗ آﮔﺮﯾﺎ ﺗﺤﺖ ﺷﺮاﯾﻂ ﮐﻨﺘﺮل ﺷﺪه در ﮔﻠﺨﺎﻧﻪ ﮐﺸﺖ ﺷﺪ. ﺑﻌﺪ از ﺗﯿﻤﺎرﻫﺎی ﺳﺮﻣﺎ، ﮔﺮﻣﺎ و ﻧﻮرزﯾﺎد، ﻧﻤﻮﻧﻪﺑﺮداری از ﺑﺮگ، ﺳﺎﻗﻪ و رﯾﺸﻪ ﺗﺤﺖ دو ﺷﺮاﯾﻂ ﮐﻨﺘﺮل و ﺗﻨﺶ ﺑﻌﺪ از اﻋﻤﺎل ﺗﯿﻤﺎر 24 و 48 ﺳﺎﻋﺖ اﻧﺠﺎم ﺷﺪ. ﺑﯿﺎن ﻧﺴﺒﯽ ژنﻫﺎی اﻧﺘﺨﺎبﺷﺪه ﺗﻌﯿﯿﻦ ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ژن stftsh5 ﺑﻌﺪ از 24 و 48 ﺳﺎﻋﺖ ﺗﺤﺖ ﺗﻨﺶ دﻣﺎی ﭘﺎﯾﯿﻦ در ﺑﺮگ و ﺳﺎﻗﻪ اﻓﺰاﯾﺶ ﺑﯿﺎن ﻣﻌﻨﯽداری را ﻧﺴﺒﺖ ﺑﻪ ﺷﺮاﯾﻂ ﮐﻨﺘﺮل ﻧﺸﺎن داد. ﻫﻤﭽﻨﯿﻦ اﯾﻦ ژن ﺗﺤﺖ ﺗﻨﺶ ﮔﺮﻣﺎﯾﯽ در دو زﻣﺎن اﻋﻤﺎل ﺗﻨﺶ در رﯾﺸﻪ و ﺑﺮگ اﻓﺰاﯾﺶ ﻣﯿﺎﻧﮕﯿﻦ ﺑﯿﺎن ﻧﺴﺒﯽ ﻧﺴﺒﺖ ﺑﻪ ﺷﺮاﯾﻂ ﮐﻨﺘﺮل داﺷﺘﻪاﺳﺖ. stftsh5 در ﺑﺮگ ﺗﺤﺖ ﺗﻨﺶ ﻧﻮر زﯾﺎد در 24 ﺳﺎﻋﺖ ﺑﻌﺪ از اﻋﻤﺎل ﺗﻨﺶ اﻓﺰاﯾﺶ ﺑﯿﺎن ﻧﺴﺒﯽ ﻧﺴﺒﺖ ﺑﻪ ﺷﺮاﯾﻂ ﻧﺮﻣﺎل ﻧﺸﺎن داد. اﻣﺎ ژن stftsh6 ﺗﺤﺖ ﺗﻨﺶ دﻣﺎی ﭘﺎﯾﯿﻦ، ﮔﺮﻣﺎ و ﻧﻮر زﯾﺎد ﺑﻌﺪ از 48 و24 ﺳﺎﻋﺖ ﺑﻌﺪ از اﻋﻤﺎل ﺗﻨﺶ در ﺑﺮگ، ﺳﺎﻗﻪ و رﯾﺸﻪ ﻣﯿﺎﻧﮕﯿﻦ ﺑﯿﺎن ﻧﺴﺒﯽ ﺑﺎﻻﯾﯽ ﺑﺮﺧﻮردار ﻧﺒﻮد. ژن stftsh10 ﺗﺤﺖ ﺗﻨﺶ ﻧﻮر زﯾﺎد در ﺳﺎﻗﻪ و رﯾﺸﻪ در 48 ﺳﺎﻋﺖ ﺑﻌﺪ از ﺗﻨﺶ اﻓﺰاﯾﺶ ﺑﯿﺎن ﻣﻌﻨﯽداری را ﻧﺸﺎن داد. در ﺑﺮگ و ﺳﺎﻗﻪ ﺗﺤﺖ ﺗﻨﺶ دﻣﺎی ﭘﺎﯾﯿﻦ در 48 ﺳﺎﻋﺖ ﺑﻌﺪ از اﻋﻤﺎل ﺗﯿﻤﺎر ﻣﯿﺎﻧﮕﯿﻦ ﺑﯿﺎن ﺑﺎﻻﯾﯽ را ﺑﻪﺧﻮد اﺧﺘﺼﺎص داد. اﻣﺎ اﯾﻦ ژن 24 و 48 ﺳﺎﻋﺖ ﺑﻌﺪ از اﻋﻤﺎل ﺗﻨﺶ ﮔﺮﻣﺎﯾﯽ و ﻧﻮر زﯾﺎد ﺑﻪﺗﺮﺗﯿﺐ اﻓﺰاﯾﺶ ﺑﯿﺎن ﺑﺎﻻﯾﯽ را در ﺳﺎﻗﻪ و رﯾﺸﻪ ﻧﺴﺒﺖ ﺑﻪ ﺷﺮاﯾﻂ ﮐﻨﺘﺮل داﺷﺖ. ﻧﺘﺎﯾﺞ اﯾﻦ ﺗﺤﻘﯿﻖ ﻧﺸﺎن داد ﮐﻪ ﺗﺤﺖ 3 ﺗﯿﻤﺎر ﺗﻨﺸﯽ )ﺳﺮﻣﺎ، ﮔﺮﻣﺎ و ﻧﻮر زﯾﺎد( ژنﻫﺎی stftsh5 و stftsh6 در ﺳﺎﻗﻪ، ﺑﺮگ و رﯾﺸﻪ ﺑﯿﺸﺘﺮﯾﻦ و ﮐﻤﺘﺮﯾﻦ ﻣﯿﺎﻧﮕﯿﻦ ﺑﯿﺎن ﻧﺴﺒﯽ را ﻧﺸﺎن داد. نتیجه گیری: در میان سه ژن، stftsh5 بالاترین بیان را در سه بافت تحت تنش سرما، گرما و نور زیاد نشان داد. با توجه به یافته های فوق، تغییرات بیشتر بیان ژن stftsh5 احتمالا می تواند ناشی از نقش موثر این ژن در تنش های غیرزیستی مخصوصا سرما، گرما و نور زیاد باشد. 

differential expression of stftsh5, stftsh6, and stftsh10 genes in potatoinduced by cold, heat, and high light stress

extended abstractintroduction and objective: abiotic stresses are among major factors limiting crop yields. cold, heat, and high-light are the most important abiotic stress among plants. thus, it is considered the understanding of molecular mechanisms response to stress.  ftsh metallopeptidase is one of the key regulators of plant response to abiotic stresses. many surveys were performed on the ftsh in different plants. however, there is not report on potato under cold, heat, and high-light stress. material and methods: in this study, the gene expression of stftsh5, stftsh6, and stftsh10 was evaluated in root, stem, and leaves under cold, heat, and high light stresses. agria genotypes were planted under controlled greenhouse conditions. after cold, heat, and high light treatment duration, leaves, stem, and root sampling were performed under two conditions stress and normal after 24h and 48h treatment. the relative expression of selected genes was estimated.results: stftsh5 gene was strongly up regulated in leaves and stem at the 24h and 48h under cold stress as compared to non-stress condition, respectively. further, this gene had the higher mean relative expression than normal condition in root and leaves at the 24h and 48h under heat stress. at 24h after high light stress, the more mean relative increase stftsh5 gene than control condition was observed. however, stftsh6 gene is not increased gene expression under low temperature, and high light after utilization of stress treatment in leaves, stem, and root. stftsh10 gene showed the significantly increased expression of in stem and root under high-light stress at the 48h. it showed an increase in gene expression in stem and leaves under cold stress at the 48h after stress treatment. however, this gene had the high gene expression in stem and root as compared to control at the 24h and 48h after stress treatment. the results of this study showed that stftsh5 and stftsh6 had the maximum and minimum gene expression in stem, leaves, and root under three stress treatments.conclusions: among three genes, stftsh5 showed the high expression in three tissues under cold, heat, and high-light stress. according to the above results, changes in gene expression of stftsh5 could refer to the key roles of this gene in abiotic stresses, particularly cold, heat, and high light stresses.