5-azacytidine enhancing expression of e-cadherin in adenocarcinoma cell line

Introduction: in this study, we assessed the expression of e-cadherin in ht29 cell line treated with 5-azacytidine and colorectal cancer patient in an iranian population. e-cadherin expression promotes metastasis and prognosis of colorectal cancer (crc). 5-azacytidine, a dna methyl transferase inhibitor, is a clinically used epigenetic drug for treatment of cancer including colorectal cancer, leading to genes activation involved in tumor suppression, especially e-cadherin.materials and methods: ht29 cell line treated with 5-azacitidine and 40 polyps, 20 tumors and 40 adjacent normal tissues samples were enrolled in this study. using the real-time pcr method, the expression levels of e-cadherin were examined in treated cell line and colorectal cancer tissue.results: this study proves that 5-azacytidine induces over expression of e-cadherin in adenocarcinoma cell line, while the expression levels of e-cadherin were not different in tumor and polyp than adjacent normal tissue.conclusion: to conclude, 5-azacytidine induces re-expression of e-cadherin in adenocarcinoma cell line. thus, 5-azacytidine as demethylation drug activated tumor suppressor gene as e-cadherin.