association of mir-200a and mir-205 expression with epithelial-mesenchymal transition in colorectal cancer cells

Introduction: micrornas play a critical role in emt regulation through tightly regulating the transcription factors. emt (epithelial-mesenchymal transition) in the colorectal cancer cell (crc) is a highly controlled mechanism, contributing to the development of progressive cancer. throughout this research, mir-200a/205 was focused as a component implicated throughout regulating the emt process in colorectal cancer cells. in this sense, the induction of the emt process was made using colorectal cancer cell lines. materials and methods: the mrna levels of e-cadherin, vimentin, β- catenin, zeb1, and snail were determined using real time-pcr for characterization of the emt process. true real time-pcr was conducted for evaluating the alteration amount of micrornas. results: the findings of this study verified the in-vitro emt model being developed. the in vitro analysis revealed a negative correlation between the zeb1 and snail mir-200a and mir205 (p=0.001) (p=0.0001). the results of mir-200a and mir205 are regulated down in vitro. conclusion: mir-200a and mir205 may be used as candidates in further research to prevent colorectal cancer's invasive properties via the emt process.