assay of bacteriorhodopsin activity and structure on polycarbonate surface by spin coating method and photochemical activity analysis

Introduction: proteinbased memory is a novel technology that employs proteins ability to participate in electronic processes. bacteriorhodopsin (br) is a membranous proton pump that it’s applications in biomolecular electronic devices has been widely studied. the results of this research show that br bounded to modified polycarbonate surface has higher activity for spin coating method.materials and methods: in an invitro study, brcontaining polymer matrix of polyvinyl alcohol and gelatin with different w/v ratios was prepared. spectroscopic and enzyme activity analysis was performed and the optimized concentration for brcontaining films was determined to be 3.2 mg/ml. brpolymer was then immobilized on the polycarbonate surface with spin coating method and afm microscopy was used to characterize brcoated polycarbonate.results: based on the obtained results we conclude that polymer concentrations below 1% significantly reduced br activity levels. a280/a570 of 3.64 for 3.2 mg/ml br solution and 4.97 for br in 1% polymer confirmed the quality prepared film. afm study of brcoated polycarbonate surface revealed the overall thickness of 25nm, indicating that we were able to prepare a surface with suitable thickness for nano electronic devicesconclusion: the results of this research show that br bounded to modified polycarbonate surface has higher activity for spin coating method.