A method of GC-MS analysis of serum metabolites

Introduction: metabolites are the intermediate and natural low molecular weight products of metabolic reactions which naturally occur within cells. metabolomics is a post-genomics study that analyzes metabolic profile in all biological samples. gas chromatography-mass spectrometry (gc- ms) is a powerful technique that detects volatile components. this study evaluates the metabolite profile of serum with a simple method for derivatization. methods: in this study, the metabolic profile of serum sample of 16 rats was analyzed using gc-ms after protein precipitation, using acetonitrile, and derivatize with a method of less in number and volume of materials. acquisition chromatograms were pre-processed and analyzed using national institute of standards and technology (nist) library.results: 35 metabolites with probability of >60% perfect match were detected according to spectrum fragments analysis by nist library. conclusion: this study represents the metabolome profile of serum instead of the change of metabolites that can be evaluated with a simple derivatization method.