Synergistic effects of serotonin and D-lys³- GHRP-6 on food intakes in food-deprived male rats

Serotonin exerts an inhibitory effect on food intakes. d-lys³-ghrp-6 is a ghrelin receptor antagonist which inhibits fasting or ghrelin- induced food intake. in the present study, the effects of serotonin on food intakes were investigated in d-lys³- ghrp-6- treated food-deprived male rats. twenty food-deprived male rats in four groups (5 in each) received third cerebral ventricular injection of saline, 10μg serotonin, 20nmol d-lys³ -ghrp-6 or combination of d-lys³-ghrp-6 and serotonin respectively. mean of one hour food intakes were measured. data was analyzed by one-way analysis of variance (anova) with post hoc tukey’s test. in all cases, p<0.05 was considered to be statistically significant. serotonin, d-lys³- ghrp-6 or combination of serotonin/d-lys³- ghrp-6 decreased the mean food intakes significantly when compared to saline. a significant decrease was observed on mean food intakes between combination of d-lys³-ghrp- 6 and serotonin group and serotonin or d-lys³- ghrp-6 groups. a significant decrease was not observed on food intakes between serotonin and d-lys³- ghrp-6 groups. serotonin and d-lys³-ghrp-6 may exert synergistic inhibitory effects on food intake in rats. decrease of the ghrelin pathway activity may have a role in mediating the inhibitory effects of serotonin on food intakes.