Radiolabeling and Bio-Distribution Study of Icd-85 With Technetium-99m As A Cancer Treatment Agent in Mice

Icd-85 is a combination of three poly-peptides, ranging from 10,000 to 30,000 dalton, derived from the venoms of an iranian brown snake (agkistrodon halys) and a yellow scorpion (hemiscorpius lepturus). labeling of this icd-85 was successfully achieved with 99mtc, through direct method using snf2 as reducing agent. labeled icd-85 was injected into mice to determine the excretion pathway. the results show that the maximum labeling yield (>75%) was obtained by using 30 μg of icd-85 in phosphate buffer (60 μl, ph 7.1) at room temperature. bio-distribution studies with radiolabeled icd-85 shows moderate clearance of the complex from blood. the improvement of the immunotherapeutic treatment of cancer requires a better knowledge of the biological actions of the icd-85 since tissue distribution studies are very important for clinical purpose.