Cyclophosphamide-induced hepatotoxicity in wistar rats: the modulatory role of gallic acid as a hepatoprotective and chemopreventive phytochemical

Background:gallic acid (ga) is an endogenous plant phenol known to have antioxidant,free radical scavenging ability,anti-inflammatory,anti-cancer,and anti-fungal properties. the aim of this study was to assess the protective effect of ga on cyclophosphamide (cpa)-induced hepatotoxicity in male wistar rats. methods: sixty rats were grouped into six groups of 10 rats per group. group 1 received distilled water. group 2 received cpa at 200 mg/kg single dose intraperitoneally on day 1. groups 3 and4 received a single dose of cpa (200 mg/kg) intraperitoneally on day 1 and then were treated with ga at 60 and 120 mg/kg body weight for 14 days,respectively. rats in groups 5 and 6 onlyreceived ga at 60 and 120 mg/kg body weight for 14 days,respectively. ga was administered orally. results:cpa induced hepatic damage as indicated by significant elevation (p < 0.05) in aspartate aminotransferase,organ weight,and evidence by the histological study. cpa also induced hepatic oxidative stress as indicated by significant elevation (p < 0.05) in malondialdehyde content,hydrogen peroxide (h2o2) generation,nitrite level,and the level of glutathione (gsh) peroxidase crashed in the cpa-treated group. ga enhanced the antioxidant defense system as indicated by significant elevation (p < 0.05) in gsh level,catalase activity,and gsh-s-transferase activity. conclusions:taken together,the result of this present study shows that ga has a protective effect on cpa-induced hepatotoxicity. © 2016 international journal of preventive medicine.