Efficacy of Co-administration of Garlic Extract and Metformin for Prevention of Gentamicin-Renal Toxicity in Wistar Rats: A Biochemical Study

Background: gentamicin (gm) nephrotoxicity has been related tooxidative stress. garlic and metformin (mf) have anti?oxadant activityand therefore, this study was aimed to evaluate the preventive andcurative effects of garlic, mf and their combination on gm indecedtubular toxicity in wistar rats.methods: in a pre?clinical study, 70 male wistar rats were randomlydesignated into 7 groups of 10 and treated as follows: group 1: receivedsaline for 20 days. group 2: were injected 100 mg/kg/d of gmintraperitoneally (ip), for 10 days and saline for 10 more days. group 3:received gm for 10 days then 20 mg/kg garlic ip for the next 10 days.group 4: received gm for 10 days and mf (100 mg/kg) orally for thenext 10 days. group 5: received gm for 10 days and a combination ofmf and garlic for the next 10 days (100 and 20 mg/kg, respectively).group 6: the same as group 5but with half?doses of mf and garlic.group 7: received gm for 10 days together with a combinationofmf and garlic. on 20th day of the experiment the serum blood ureanitrogen (bun) and creatinine (cr) were measured and compared indifferent groups.results: gm injection significantly increased the serum bun andcr (p < 0.05). administration of mf, garlic or their combination with orafter injection of gm (high doses) could atenuate bun and cr.conclusions: the results indicate that mf and garlic or their combinationhave curative and protective activity against gm nephrotoxicity.