Effect of Continues Training and High Intensity Interval Training on miR-29a and CTGF Gene Expression in Male Wistar Diabetic Rats’ Heart Tissue

Objective: high-intensity interval training (hiit) and continues aerobic training (ct) have cardio-protective effects in diabetic rats. the functional role of microrna in heart was studied. only mir-29a levels were found to correlate with cardiac fibrosis, this study tests the hypothesis that applying hiit and ct cases mir-29a increasing is associated with a reduction of connective tissue growth factor (ctgf) induced cardiac fibrosis. materials and methods: in this randomized controlled trial, 18 male diabetic rats were included. they were divided into 3 groups called as hiit, ct and control. exercise protocol was performed 5 days/week for 5 weeks. the mir-29a and ctgf synthesis were compared between the groups by real time- pcr. results: our results demonstrated that elevation of mir-29a using hiit (2.67±1.02, p=0.010,) or ct (1.79±0.49, p=0.002) are effective in inhibiting ctgf (hiit:0.17±.0. 07,, p=0.000- ct:0.39±0.27,p= 0.000 -induced cardiac fibrosis, suggesting that these types training would be selected as a new adjunctive therapy in the heart fibrosis- derived diabetic. discussion: the hiit and ct showed increased levels of mir- 29a compared ct group which is shown to decrease ctgf level resulting into lowered fibrosis of heart tissue in diabetic patients.