cinnamon extract changes the expression of mirnas26-b, 29a, 223 and320 in insulin resistant adipocytes

Objective: insulin resistance (ir) is the major cause in type 2 diabetes mellitus (t2dm). expression of some mirnas can be changed in response to a drug treatment for ir, and used as the biomarker in ir. this study set out to determine the effect of cinnamon extract (cinnamaldehyde) on some mirnas expression in ir adipocytes.materials and methods: in this invitro study the 3t3l1 cells were expanded in dulbecco rsquo;s modified eagle rsquo;s medium (dmem), differentiated into adipocytes phenotype and insulin resistant with high glucose medium, then the cells were treated with cinnamaldehyde. to determine of the mirnas profiling in 3t3l1 adipocytes, insulinresistant adipocytes and treated insulinresistant adipocytes quantitative realtime pcr method was performed.results: ir adipocytes exhibited a significantly increase in mirs 29a, 223 and 320 expression, and decrease in mir26b expression in compare to the normal adipocytes(p-value<0.001 and p-value= 0.024 respectively). however in response to cinnamaldehyde in ir adipocytes, expression of mirs 29a, 223 and 320 were downregulated while expression of mir26b was upregulated neared it to the normal level (p-value= 0.003 and p-value= 0.002 respectively).conclusion: ir changes expression of intended mirs, so that cinnamaldehyde treatment helps to improve and normalize the changes. cinnamon as the herbal product can be helpful for ir particular in adipose tissue.