Design of nano structured intelligent surface for Harvesting Epithelial cells

Poly n-isopropylacrylamide was successfully grafted onto a polystyrene cell culture dish by gamma ray. in this study, the effect of a radiation dose (radiation absorbed dosage of 40 kgy) under appropriate temperature and grafting conditions was investigated. the ftir analysis showed the existence of the graft pnipaam on the substrate. the sem and afm images clearly confirmed this claim. surface topography and graft thickness in afm images of the radiated samples showed that the pnipaam at the absorbed dose of radiation was properly grafted. the thickness of these grafts was about 60 nm. the drop water contact angles of the best grafted sample at 37°c and 10°c were 55.3 ± 1.2o and 61.2 ± 0.9o respectively, which showed the hydrophilicity and hydrophobicity of the grafted surfaces. thermo-responsive polymers were grafted to dishes covalently which allowed epithelial cells to attach and proliferate at 37°c; the cells also detached spontaneously without using enzymes when the temperature dropped below 32°c. this characteristic proves that this type of grafted material has potential as a biomaterial for cell sheet engineering.