تاثیر ریزوباکتر های محرک رشد بر فعالیت آنزیم های آنتی اکسیدان، خصوصیات فیزیولوژیکی و رشد ریشه چهار رقم نخود (cicer arietinum l.) در شرایط دیم استان ایلام

به منظور بررسی اثر ریزوباکتر ها محرک رشد بر فعالیت آنزیم های آنتی اکسیدان، خصوصیات فیزیولوژیکی و رشد ریشه چهار رقم نخود در شرایط دیم استان ایلام، آزمایشی به صورت فاکتوریل در قالب طرح بلوک های کامل تصادفی با سه تکرار در سال زراعی94-1393 در ایستگاه مرکز تحقیقات کشاورزی سرابله اجرا گردید. تیمار های آزمایش شامل سه رقم نخود (هاشم، آزاد و آرمان) و توده محلی و ریزو باکتر ها محرک رشد و کود شیمیایی نیتروژن (عدم مصرف کود نیتروژن، 10‌کیلوگرم کود نیتروژن، 20‌کیلوگرم کود نیتروژن، آزسپریلیوم برازیلنس (azospirillum brasilense)+ عدم مصرف کود شیمیایی نیتروژن، آزسپریلیوم برازیلنس+10‌کیلوگرم کود شیمیایی نیتروژن، آزسپریلیوم برازیلنس+20‌کیلوگرم کود شیمیایی نیتروژن، ازتوباکتر کروکوم (azotobacter chroococcum)+ عدم مصرف کود شیمیایی نیتروژن، ازتوباکتر کروکوم+10‌کیلوگرم کود شیمیایی نیتروژن و ازتوباکتر کروکوم+20‌کیلوگرم کود شیمیایی نیتروژن) درنظرگرفته شدند. اثر برهمکنش رقم و ریزو باکتری ها محرک رشد بر خصوصیات فیزیولوژیکی و فعالیت آنزیم‌های آنتی اکسیدان و صفات ریشه معنی‌دار بود. استفاده از ریزو باکتری ها محرک رشد میزان رنگیزه های فتوسنتزی و محتوای آب نسبی را افزایش و موجب کاهش مالون‌دی‌آلدئید گردید. استفاده از این ریزو باکتر ها در تمامی ارقام مورد استفاده موجب افزایش فعالیت آنزیم های آنتی اکسیدان و همچنین موجب افزایش سیستم ریشه گردید. در این پ‍ژوهش ارقام هاشم و آزاد به آزسپریلیوم و رقم آرمان و توده محلی به ازتوباکتر واکنش بهتری نشان دادند. رقم آزاد به‌علاوه آزسپریلیوم + 10‌کیلوگرم کود نیتروژن دارای بیشترین میزان کلروفیلa و b، محتوای آب نسبی، فعالیت آنزیمی سوپراکسید دیسموتاز، پرکسیداز، حجم ریشه، وزن‌خشک ریشه و طول ریشه بود و توده محلی به‌علاوه تیمار شاهد (عدم مصرف کود نیتروژن) کمترین فعالیت آنزیم های آنتی اکسیدان، خصوصیات فیزیولوژیکی و رشد ریشه را در شرایط دیم دارا بود.

The effect of Plant Growth Promoting Rhizohacteria on activities of antioxidative enzymes, physiological characteristics and root growth of four chickpea (Cicer arietinum L.) cultivars under dry land conditions of Ilam privince

Introduction;Chickpea is widely cultivated as an important cool season grain legume crop throughout the world. According to FAO, Iran is one of the major chickpea (Cicer arietinum L.) producing countries in the world. In Iran, chickpea is the most important pulse crop with respect to production and area under cultivation. This crop is cultivated in about 500,000 ha, of which over 95 percent are grown under rainfed conditions. Average chickpea yield in Iran is about 400 to 600 kg.ha−1, that is well below the world average of 900 kg.ha−1. Drought and high temperature are two major factors limiting the growth and productivity of chickpea during summer in many regions. Drought stress is common in many parts of the world and more than 50% of the globe is arid or semi arid and plants are subjected to some level of drought stress. Drought stress can adversely affect plant growth and production. Plant response to drought stress, at cellular and molecular level, limits plant growth and yield. It has been shown that several PGPR can support plants by producing antioxidant factors or modulate photosynthesis decreasing ROS and thus lowering the need for antioxidant activity during stress which could explain why primed plants tend to decrease their own antioxidant defense system. Over reduction of the photosynthetic electron transport chain induces the generation of reactive oxygen species (ROS) such as singlet oxygen (1O2), superoxide anion (O2•), hydrogen peroxide (H2O2), and hydroxyl radical (•OH). Therefore, the decline in growth and productivity due to these stress factors is associated with increased levels of ROS, which cause damage to cellular structures and macromolecules. In order to maintain or increase crop productivity it becomes necessary to evolve efficient lowcost technologies for abiotic stress management. It is now a priority area research for developing strategies to cope with abiotic stresses including development of stress tolerant varieties, shifting crop calendars, resource management practices etc. However, most of these techniques are costintensive and time taking. Recent studies indicate that soil microorganisms can help crops withstand abiotic stresses more efficiently. These include tolerance to salt and water stress (Azospirillum sp., Pseudomanas sp, Bacillus sp). The increased H2O2 content under stress conditions led to lipid peroxidation, which is widely used as an indicator of stressinduced oxidative damage. The relative water content (RWC) and lower electrolyte ion leakage (EL) in plants exposed to drought has been considered indicative of a relative tolerance to water stress. In our study, RWC declined while %EL increased in both inoculated and uninoculated seedlings under drought stress compared to normal irrigation. However, bacterial inoculation did help plants to increase their RWC and to decrease their %EL as compared with uninoculated plants in drought stress. Investigations involving wheat species and varieties have detected increases in the activity of superoxide dismutase (SOD), catalase (CAT), and nonspecific peroxidase (guaiacol peroxidase, POD). The main objective was to evaluate the effects of Plant Growth Promoting Rhizohacteria on activities of antioxidative enzymes, physiologic traits and root growth of chickpea in dry land conditions of Ilam province.; ;Materials Methods;To evaluate the effect of Plant Growth Promoting Rhizohacteria on activities of antioxidative enzymes, physologiceal charactersitices and root growth of chickpea under dry land conditions of Ilam province, an experimental field was conducted using factorial arrangement based on randomized complete block design with three replications at Agricultural Research Research Center of Ilam during 20142015. Studied factors included cultivars (Azad, Hashem, Arman and locallandrace) and Plant Growth Promoting Rhizohacteria (without inoculation, 10 kg nitrogen, 20 kg nitrogen, Azospirillum + without nitrogen, Azospirillum + 10 kg nitrogen, Azospirillum + 20 kg nitrogen, Azetobacter + without nitrogen, Azetobacter + 10 kg nitrogen, Azetobacter + 20 kg nitrogen. Cultivars were sown on 16 November, 2013. Eeight rows with 30 cm width and 4 m long were designed during the growth season, hand weeding was done in necessary times. Studied traits were included of chlorophyll a and b, RWC, MAD, SOD, POD, CAT, root volum, dray root weight and main root length. The data were analyzed statistically by SAS program and the data means were compared by Duncan apos;s multiple range test (DMRT).; ;Results Discussion;The interaction effect between cultivars× PGPR on chlorophyll a and b, RWC, MAD, SOD, POD, CAT, root volume, dry root weight and main root length were significant. Application of nitrogen and PGPR in different cultivars provided better nutrition condition for plant growth by reducing reactive oxygen species (ROS) because these bacteria need these elements to grow and development. PGPR inoculation significantly increased the contents of chlorophyll a and b, RWC and decresed MAD content in chickpea plants. PGPR improved water status, enhance its defense system, and alleviate oxidative damage caused by drought stress. Drought stress damage decrease, evaluated as MDA content, has been observed under different stress conditions in PGPR. The improved plant growth under dry land farming was also observed in chickpea by inoculation of PGPR and application of N, which was found to be associated with enhanced, root system in field grown under rainfed condition.; ;Conclusion;Under dry land condition, due to the generation of reactive oxygen species, an efficient antioxidant system is needed in the plant. It has been observed that PGPR increase the activity of antioxidant enzymes of host plants. Study conducted on chickpea under dry land conditions showed that PGPR enhanced the activities of antioxidant enzymes such as superoxide dismutase, peroxidase and catalase compared to those in uninoculated control plants.