مقایسه تاثیر لیزین‌هیدروکلراید و بایولیز‌سولفات بر عملکرد، پاسخ‌‌ایمنی، فراسنجه‌های خونی و استخوانی جوجه‌های گوشتی

در آزمایش حاضر تاثیر بایولیز سولفات در مقایسه با لیزین هیدروکلراید بر عملکرد، پاسخ ایمنی، فراسنجه های خونی و استخوانی جوجه های گوشتی مورد مقایسه قرار گرفت. تعداد 200 قطعه جوجه گوشتی سویه راس308 (مخلوط مساوی نر و ماده) در قالب طرح کاملاً تصادفی به پنج تیمار دارای پنج تکرار و 8 قطعه در هر تکرار اختصاص داده شد. تیمارهای آزمایشی عبارت بودند از: 1) 100 درصد لیزین مکمل جیره از اللیزین هیدروکلراید تامین شد (جیره پایه یا شاهد)، 2) 75 درصد لیزین مکمل جیره توسط اللیزین هیدروکلراید و 25 درصد توسط بایولیز سولفات تامین شد، 3) 50 درصد لیزین مکمل جیره توسط اللیزین هیدروکلراید و 50 درصد توسط بایولیز سولفات تامین شد، 4) 25 درصد لیزین مکمل جیره توسط اللیزین هیدروکلراید و 75 درصد توسط بایولیز سولفات تامین شد، 5) 100 درصد لیزین مکمل جیره توسط بایولیز سولفات تامین شد. طی دوره 421 روزگی، مصرف خوراک و وزن بدن جوجه های تغذیه شده با جیره حاوی سطح 100 درصد بایولیز سولفات نسبت به وزن بدن جوجه های گروه شاهد بیشتر بود و جوجه های تغذیه شده با جیره حاوی سطوح 25 و 75 درصد بایولیز سولفات ضریب تبدیل خوراک بالاتری داشتند (0.05 p <). تیتر آنتی بادی علیه آنفلوآنزا، درصد کلسیم و فسفر، طول، وزن و شاخص استخوان در جوجه های تغذیه شده با جیره حاوی سطح 100 درصد بایولیز سولفات نسبت به مقادیر جوجه های گروه شاهد بیشتر بود (0.05 p <). به طور کلی تامین کل لیزین جیره جوجه‌های گوشتی از بایولیز سولفات در مقایسه با اللیزین هیدروکلراید سبب افزایش خوراک مصرفی، بهبود پاسخ ایمنی و فراسنجه های استخوانی جوجه‌های گوشتی شد ولی بر وزن بدن تاثیر نداشت. با وجود قیمت کمتر بایولیز سولفات، گنجاندن آن در جیره منجر به کاهش نسبت درآمد به هزینه شد و از لحاظ اقتصادی به صرفه نیست.

Comparing Effect of Lysine-HCl and Biolys-Sulfate on Broiler Chickens Performance, Immune Response, Blood Metabolites and Bone parameters

Introduction Commercially available feedgrade crystalline amino acids have provided an opportunity for nutritionists to formulate low CP costeffective diets while maintaining optimal protein utilization by birds. Lysine is the first limiting amino acid in practical diets for broilers when soybean meal is not the major protein source. Lysine supplementation under such circumstances almost becomes unavoidable in particular when dietary CP contents are to be reduced and when using the concept of ideal amino acid ratio in which lysine is used as a reference amino acid. LLysine HCl, which contains a minimum of 78% lysine is the dominant source of lysine for addition to poultry diets. LLysine sulfate products have minimum lysine contents of 46.8% in Biolys60 (Degussa AG, Frankfurt, Germany) or 51% in Lysine Plus (CJ Biotech Co. Ltd., Liaocheng, Shandong, China). A recent study reported that the bioefficacy of Llysine sulfate relative to Llysine HCl was similar when using daily gain and feed conversion of young pigs as the response criteria. In addition, several other swine and broiler experiments had been conducted to compare the bioefficacy of lysine in Llysine sulfate and Llysine HCl. Most of these studies used animal performance as the main response variable. However, very little research has been conducted to determine the effects of different lysine sources on plasma metabolites and bone characteristics of broiler chickens as the response variable. Therefore, the present study was conducted to examine the comparative effects of Llysine sulfate and Llysine HCl in broiler chickens using growth performance, blood metabolites and bone parameters as response variables.Materials and Methods In this experiment compared the effects of LLysinehydrochloride (Lys) and BiolysSulfate (BioLys) on broiler chickens’ performance and Immune Response. In a completely randomized design, 200 Ross308 broiler chickens were allocated to 5 dietary treatments with 5 replicates and 8 birds in each. Dietary treatments including: 1 Control  or basal diets (100% of supplemented Lys from LysHCl), 2 Ration 25:75 (75% of supplemented Lys from LysHCl and 25% from BioLysSulfate), 3 Ration 50:50 (50% of supplemented Lys from LysHCl and 50% from BioLysSulfate), 4 Ration 75:25 (25% of supplemented Lys from LysHCl and 75% from BioLysSulfate) and 5 Ration 100 (100% of supplemented Lys from BioLysSulfate). Feed ingredients (i.e., corn, corn gluten meal, and soybean meal) were analyzed for amino acid by NIRS in Evonik company representative in IRAN. The composition of basal diet and nutrient composition of feed ingredients. During the study, feed intake (FI) and body weight (BW) were determined at the end of starter, grower and finisher periods, and mortalitycorrected FCR was calculated by accordingly. European production efficiency factor (EPEF) was calculated as: EPEF = [Viability (%) × BW (kg) / age (d) × FCR (kg feed/kg gain)] × 100.All the birds were vaccinated intramuscularly through breast muscle with killed Newcastle disease and influenza vaccine (Cevac: New FluKem) at 7 d of age, and Newcastle disease La Sota vaccine was used in the drinking water for vaccination at 14 d of age. Antibody titer against Newcastle disease, avian influenza and sheep red blood cell were detected. At the end of the experiment (42 d of age), 2 blood samples from the wing vein of 2 birds (almost near to mean body weight of each pen) were collected and blood metabolites like serum glucose, triacylglycerol, total cholesterol, albumin, uric acid and creatinine were detected. The other blood sample was used to detecting heterophil and lymphocyte percentages and heterophil to lymphocyte ratio accordingly. Finally 1 broiler from each replicate were selected and slathered, then tibia bone parameters like height, width, diameter, Ca and P percentage were detected. Results and Discussion The results showed that from 142 d, broiler chickens fed the 100diet had higher feed intake and body weight gain as compared to control group (P < 0.05). Dietary treatments had no effects on serum cholesterol, glucose and uric acid concentrations, spleen and thymus relative weight, heterophil and lymphocyte percentages and heterophil to lymphocyte ratio (H:L), antibody titter against SRBC and NewCastle diseases, tibia bone diameter and width, dry matter and ash percentages (P > 0.05). As compared to control group, broiler chickens fed diet containing 100% BioLys had higher serum total protein concentration, antibody titter against avian influenza and bone Ca and P percentages, tibia bone length, width and index (P < 0.05).Conclusion In conclusion, inclusion of BioLysSulfate rather than LLysineHCl to broiler chickens diet, increased broiler chickens feed intake and improved immune response and bone parameters but had no effect on broiler chickens body weight. In spite of cheaper cost of BioLysSulfate, its inclusion in broiler chickens diet decreased income over feed cost ratio and its use is not economically advisable.