Development of new Populus euphratica Oliv. Clones, using somaclonal variation

The method of somaclonal variation was used to induce genetic variation in populus euphratica oliv.using on long term callus culture. callus was induced by culture of naturally pollinated ovaries into the ms media supplemented different combinations and concentration of 2a-d and kinetin growth regulators hormone. significant differences were observed between growth regulator hormones for callus induction at 0.01 levels. callus initiation was maximal (average h::.5'~'n of cultured ovaries produced callus) in ms medium containing 2 mg/i 2, 4-d und 0.1 mg/l kinetin. plantlets were observed from l2 months old green calli following red pigment initiation. ms medium containing 2mg/1 ba and 0.5mg/1 naa was the most favorable for plantlets regeneration. two plantlets nut of nearly 400 regenerants showed highly morphological variation. variants were noticed in growth habit and leaf shapes both juvenile stages in a pot and at mature stage of tree in farm level.