ساخت و بررسی ویژگی‌های داربست اسفنجی دولایه ساخته شده از نانوسلولز برای مهندسی بافت پوست

ﻫﺪف از اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺳﺎﺧﺖ دارﺑﺴﺖ دوﻻﯾﻪ ﺣﺎوی ﻧﺎﻧﻮاﻟﯿﺎف ﺳﻠﻮﻟﺰ/ﭘﻠﯽوﯾﻨﯿﻞ اﻟﮑﻞ (cnf/pva) ﺑﺮای ارزﯾﺎﺑﯽ ﻗﺎﺑﻠﯿﺖ اﺳﺘﻔﺎده آن در ﻣﻬﻨﺪﺳﯽ ﺑﺎﻓﺖ ﭘﻮﺳﺖ ﺑﻮده اﺳﺖ. ﯾﮏ روش ﭘﯿﻮﺳﺘﻪ ﺑﺮای اﺿﺎﻓﻪ ﮐﺮدن ﻻﯾﻪﻫﺎ ﺑﺎ دو ﻏﻠﻈﺖ ﻣﺨﺘﻠﻒ از ﭘﻠﯿﻤﺮﻫﺎ ﺗﻮﺳﻂ روش ﺧﺸﮏ ﮐﺮدن اﻧﺠﻤﺎدی ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﮔﺮﻓﺖ. آﻧﺎﻟﯿﺰ fe-sem ﻧﺸﺎن داد ﮐﻪ دارﺑﺴﺖﻫﺎی ﺑﺪﺳﺖ آﻣﺪه ﺗﺨﻠﺨﻞ ﺑﻪ ﻫﻢﭘﯿﻮﺳﺘﻪ و اﻧﺪازه ﻣﻨﺎﻓﺬی دارﻧﺪ ﮐﻪ در ﻏﻠﻈﺖ ﮐﻤﺘﺮ ﭘﻠﯿﻤﺮﻫﺎ، اﻓﺰاﯾﺶ ﻣﯽﯾﺎﺑﻨﺪ. ﻫﻤﭽﻨﯿﻦ دارﺑﺴﺖﻫﺎ ﺑﺮاﺳﺎس ﺟﺬب آب، وﯾﮋﮔﯽﻫﺎی ﻣﮑﺎﻧﯿﮑﯽ و آزﻣﻮن mtt ﻃﺒﻘﻪﺑﻨﺪی ﺷﺪﻧﺪ. ﻏﻠﻈﺖ ﮐﻤﺘﺮ ﭘﻠﯿﻤﺮﻫﺎ، ﺟﺬب آب را اﻓﺰاﯾﺶ و ﻣﻘﺎوﻣﺖ ﻣﮑﺎﻧﯿﮑﯽ را ﮐﺎﻫﺶ داد. ﻧﺘﺎﯾﺞ mtt ﻧﯿﺰ ﻧﺸﺎن داد ﮐﻪ اﯾﻦ دارﺑﺴﺖﻫﺎی ﻧﺎﻧﻮﻓﯿﺒﺮی ﻣﯽﺗﻮاﻧﻨﺪ ﻧﯿﺎزﻣﻨﺪیﻫﺎی ﻻزم ﺑﺮای اﺳﺘﻔﺎده در ﻣﻬﻨﺪﺳﯽ ﺑﺎﻓﺖ را داﺷﺘﻪ ﺑﺎﺷﻨﺪ.

Fabrication of Bilayer Nanocellulosic Aerogel Scaffold and Evaluation of Its Properties for Skin Tissue engineering

This study aimed to fabricate the bilayer scaffold containing nanocellulose/poly (vinyl) alcohol (CNF/PVA) to evaluate its potential use as a mimic for skin tissue engineering. A continuous process of layer addition with two different concentrations of polymers by freezedrying technique was used. FESEM analysis indicated that the obtained scaffolds had interconnected porosity and pore size, which increased in lower concentration of polymers. The scaffolds were also characterized by water uptake, mechanical properties and MTT assay. Lower concentration of polymers increased water uptake and decreased mechanical strength. The MTT assay results showed that these nanofibrous scaffolds meet the requirement as a biodegradable material for skin repair.