effect of iron-amino acid chelates on antioxidant capacity and nutritional value of soybean

Abstract introduction:supplying a sufficient amount of available iron (fe) for plant growth in hydroponic nutrient solutions is a great challenge. there are various fe fertilizers to eliminate iron deficiency in crops. the chelators commonly used to supply fe in nutrient solutions have several disadvantages and may negatively affect plant growth. the objective of the current paper was to evaluate the effects of some various fe chelates, (fearginine, feglycine, fehistidine, felysine, feglutamine and feedta) on the physiological properties and nutraceutical potential of soybean (glycine max (l.) merr.) grown in nutrient solution. materials and methods:the experiments had a randomized complete block design with three replications and the treatments were arranged in factorial combination. the plants were grown in a greenhouse of faculty of agriculture at lorestan university in 2018. in this study, fe chelates were synthesized. then, soybean seeds were sterilized and germinated in 11 μm filter paper. sevenday seedlings were transplanted into perlite and watered regularly with distilled water for a week. then, twopair leaflet seedlings were placed in continuously aerated solution under controlled conditions of the greenhouse. six different iron treatments (at fe level=50 µm) were applied including: feedta, fearginine, feglycine, fehistidine, feglutamine, and felysine. the plant leaves were collected at 10leaves stages. the seed collection was done in the maturity of harvest (r8). afterwards, physiological parameters, antioxidant enzymes activity and seeds quantity and quality were measured. results and discussion:feamino acid application significantly (p£0.05) enhanced root and shoot dry matter yield, total chlorophyll content, 1000 seed weight, seed yield, seed protein content, seed oil content, oleic acid, and number of seeds in each pod in comparison with feedta treatment. the maximum chlorophyll content, seed protein and seed oil were observed in the feglycine treatment (increased by 66.25%, 103.48% and 85.11%, respectively compared to that of control). concentrations of ferritin, iron, zinc, and nitrogen in soybean seeds were also higher in feamino acid chelate treatments compared to feedta. the effect of feamino acid chelates on the fe content in seeds was in the order feglycine> feglutamine> felysine> fehistidine>  fearginine. catalase activity (ec 1.11.1.6)and ascorbate peroxidase (ec 1.11.1.11) significantly (p£0.05) increased in all feamino acid chelates treatments compared to feedta treatment. conclusion: according to the results, feamino acid chelates could provide the required amount of iron to soybean and this resulted in seed quality enhancement. therefore, feamino acids can be used as more efficient fertilizers than feedta in nutrient solution, and they can be used as an alternative to feedta to supply fe in nutrient solutions.

Effect of Iron-Amino Acid Chelates on Antioxidant Capacity and Nutritional Value of Soybean

ABSTRACT Introduction:Supplying a sufficient amount of available iron (Fe) for plant growth in hydroponic nutrient solutions is a great challenge. There are various Fe fertilizers to eliminate iron deficiency in crops. The chelators commonly used to supply Fe in nutrient solutions have several disadvantages and may negatively affect plant growth. The objective of the current paper was to evaluate the effects of some various Fe chelates, (Fearginine, Feglycine, Fehistidine, Felysine, Feglutamine and FeEDTA) on the physiological properties and nutraceutical potential of soybean (Glycine max (L.) Merr.) grown in nutrient solution. Materials and Methods:The experiments had a randomized complete block design with three replications and the treatments were arranged in factorial combination. The plants were grown in a greenhouse of Faculty of Agriculture at Lorestan University in 2018. In this study, Fe chelates were synthesized. Then, soybean seeds were sterilized and germinated in 11 μm filter paper. Sevenday seedlings were transplanted into perlite and watered regularly with distilled water for a week. Then, Twopair leaflet seedlings were placed in continuously aerated solution under controlled conditions of the greenhouse. Six different iron treatments (at Fe level=50 µM) were applied including: FeEDTA, Fearginine, FeGlycine, Fehistidine, Feglutamine, and Felysine. The plant leaves were collected at 10leaves stages. The seed collection was done in the maturity of harvest (R8). Afterwards, physiological parameters, antioxidant enzymes activity and seeds quantity and quality were measured. Results and Discussion:Feamino acid application significantly (P£0.05) enhanced root and shoot dry matter yield, total chlorophyll content, 1000 seed weight, seed yield, seed protein content, seed oil content, oleic acid, and number of seeds in each pod in comparison with FeEDTA treatment. The maximum chlorophyll content, seed protein and seed oil were observed in the Feglycine treatment (increased by 66.25%, 103.48% and 85.11%, respectively compared to that of control). Concentrations of ferritin, iron, zinc, and nitrogen in soybean seeds were also higher in Feamino acid chelate treatments compared to FeEDTA. The effect of Feamino acid chelates on the Fe content in seeds was in the order Feglycine> Feglutamine> Felysine> Fehistidine>  Fearginine. Catalase activity (EC 1.11.1.6)and ascorbate peroxidase (EC 1.11.1.11) significantly (P£0.05) increased in all Feamino acid chelates treatments compared to FeEDTA treatment. Conclusion: According to the results, Feamino acid chelates could provide the required amount of iron to soybean and this resulted in seed quality enhancement. Therefore, Feamino acids can be used as more efficient fertilizers than FeEDTA in nutrient solution, and they can be used as an alternative to FeEDTA to supply Fe in nutrient solutions.