Histopathological evaluation of the effect of platelet-rich fibrin on canine cutaneous incisional wound healing

Platelets are a natural source of growth factors and cytokines that promote wound healing, and platelet-rich fibrin contains concentrated growth factors. growth factors in platelet-rich fibrin may promote wound repair. in this study the effect of platelet-rich fibrin was evaluated in a dog model of cutaneous incisional wounds using histology and semi-quantitative evaluation. a pair of ten-centimeter full-thickness parallel linear cutaneous wounds were created on the backs of 15 dogs on the both sides of vertebral column. on the left side the platelet-rich fibrin clot was applied to the edges of the wound (treatment group) and the right side received nothing (control group). all wounds were then closed with 3-0 non-absorbable nylon suture. the dogs were divided into three groups of five dogs. the wound tissues were sampled by electrosurgery in group one after 3 days, group two after 7 days and group three after 14 days post surgery. for each specimen, histopathological examination and semi-quantitative evaluation was performed by light microscopy using hematoxylin & eosin and masson’s trichrome staining. the results demonstrated that platelet-rich fibrin improved and accelerated cutaneous incisional wound healing.