investigation of bacterial contamination with klebsilla and e. coli in the prepucal cavity of pubertal and adult age in caprine

The herein research was carried out in order to identified the presence of bacteria inside prepuce cavity of male caprine in both mature and pubertal age with focusing on klebsilla and e coli species. eighty prepuce swabs (fifty form mature and thirty from pubertal age) before slaughtering and cultured on blood agar and nutrient agar, bacterial isolation were identified with biochemical teats and finally by pcr. the present study found a significant difference (p < 0.01) between the prepuce swabs from caprine mature age (64%) and pubertal age (40%). six various microorganisms were detected in prepuce samples in mature age, while four types were isolated from pubertal age. positive isolation swabs detected the presence of staphylococcus aurues, escherichia coli, pseudomonas aeruginosa and streptococcus faecalis in both ages. proteus mirabilis and klebsiella pneumonia was isolated from mature age only. significant isolation (p < 0.01) was appear of escherichia coli among all different bacterial types. this research deduce that there was that the presence of bacteria inside prepuce of male genital system in both mature and pubertal age and their where a balance between genital immunity and localization of these bacteria and any stress factor may be lead to infection with such microbes, more over the mature male had more bacterial types due to the male matting behavior, finally the e. coli normally found in prepuce cavity as a normal flora of both ages and the klebsilla species also found in mature age as a non-specific bacterial types.