Evaluation of antibody against hydatid fluid, protoscolex and adult worms of Echinococcus granulosus antigens by ELISA in mice

Echinococcosis is one of the world’s relevant zoonoses, affecting both humans and theirdomestic animals and caused by infection with the metacestode stage of the dog tapeworm,echinococcus granulosus. in the present study, twenty mice were randomly divided into 4groups of 5. each mouse in groups 1, 2 and 3 was immunized with 100 μg of hydatid cystfluid protein, protoscolex and adult worms of e. granulosus proteins in 100 μl of phosphatebufferedsaline which was emulsified with an equal volume of freund’s complete adjuvantrespectively. in group 4, mice were just immunized with above adjuvant in pbs. mice wereboosted four weeks after the first immunization with the same preparation and with freund’sincomplete adjuvant. three weeks later, each mouse was challenged with 2000 protoscolicesintraperitoneally. sera were collected before and after each immunization and serumantibodies were tested by elisa. results showed that the level of antibody in mice whichwere immunized with adult worms of e. granulosus on day 28 was roughly seven timeshigher than before immunization and was higher than hydatid cyst fluid and protoscolex.level of antibody in mice immunized with adult worms of e. granulosus was also higher thanin hydatid cyst fluid and protoscolex groups at day 49. the results of this article indicate thatantigen of adult worms of e. granulosus can be used as a candidate for production of avaccine.