Optimized and validated spectrophotometric methods for the determination of amiodarone hydrochloride in commercial dosage forms using N-bromosuccinimide and bromothymol blue

Two optimized and validated spectrophotometric methods have been developed for the determination of amiodarone hydrochloride in commercial dosage forms. method a is based on the reaction of tertiary amino group of the drug with n-bromosuccinimide in methanol–acetone medium resulting in the formation of a yellow colored product,which absorbed maximally at 353 nm. method b involves the formation of a colored chloroform extractable ion-pair complex of the drug with bromothymol blue at ph 2.32 absorbing maximally at 400 nm. beer's law is obeyed in the concentration ranges 50–600 and 2–55 μg ml−1 with molar absorptivity of 1.58 × 102 and 1.50 × 104 l mol−1 cm−1 for methods a and b,respectively. the regression analysis yields the calibration equations: a = 4.012 × 10−4 + 1.16 × 10−3 c and a = 9.612 × 10−4 + 2.198 × 10−2 c for methods a and b,respectively. the application of the proposed methods to commercial dosage forms is presented. © 2013