Biochemical studies on antibiotic production from Streptomyces sp.: Taxonomy,fermentation,isolation and biological properties

Tunicamycin is a nucleotide antibiotic which was isolated from the fermentation broth of a streptomyces strain no. t-4. according to the morphological,cultural,physiological and biochemical characteristics,and 16s rdna sequence analysis,strain t-4 was identified as streptomyces torulosus. it is active in vitro against some microbial pathogenic viz: staphylococcus aureus,nctc 7447; micrococcus lutea,atcc 9341; bacillus subtilis,nctc 10400; b. pumilus,nctc; klebsiella pneumonia,ncimb 9111; escherichia coli,nctc 10416; pseudomonas aeruginosa,atcc 10145; saccharomyces cerevisiae atcc 9763; candida albicans,imru 3669; aspergillus flavus,imi 111023; aspergillus niger imi 31276; aspergillus fumigatus atcc 16424; fusarium oxysporum; rhizoctonia solani; alternaria alternata; botrytis fabae and penicillium chrysogenium. the production media were optimized for maximum yield of secondary metabolites. the metabolites were extracted using n-butanol (1:1,v/v) at ph 7.0. the chemical structural analysis with uv,ir,and ms spectral analyses confirmed that the compound produced by streptomyces torulosus,t-4 is tunicamycin antibiotic. © 2011.