Distribution of fatty acids in triacylglycerols from ungrafted Desi Mango (Mangifera indica) kernel lipids

The lipids of ungrafted ‘desi’ mango (mangifera indica) kernel were extracted with chloroform/methanol, purified and triacylglycerols (82.6%) were separated by thin layer chromatography (tlc). the pure triglycerides are fractionated into five bands by 25% silver nitrate-impregnated thin layer chromatography (ag-tlc). the composition and position of fatty acids at a, a0 and b-positions of fractionated/unfractionated triacylglycerols were determined by the use of pancreatic lipase, sodium tetraborate-impregnated thin layer chromatography (borate-tlc) and gas liquid chromatography (glc). the highest percentage of the most saturated band was 55.6% which comprised of sn-1,3-disaturated-2-oleoglycerols and sn-1(3)saturated- 2,3(1,2)-dioleoylglycerols. the oleic, linoleic and linolenic acids occupied β-position depending upon comparatively higher percentage of unsaturated fatty acids in specific fraction.