Comparative study of amyloidogenic potential of AgNO3 and Freund's Adjuvant (AF) with that of Vitamin Free Casein, on Spatio-Temporal Pattern of Experimental Amyloidosis in Mice

Reactive amyloidosis is a condition that complicates a long list of chronic inflammation, chronic infectious, malignant, and hereditary disorders. in the present study the potential effects of two amyloidogenic substances: ie. agno3 and freund's adjuvant (af) with that of vitamin free casein, on spatio-temporal pattem of experimental amyloidosis in mice were compared. for this purpose atotal of 40 male swees mice, obtained from pasteur institute tehran, after being weighted were randomly divided into 4 groups including 2 treatments, 1 control (vitamin freecasein) and 1 positive control (normal saline) . at theendof 3rd, 5thand 7th weeks of experiment 3 mice were randomly selected and euthnised. spleen sample of eachanimal obtained andpreserved in 101. neutral bufferformalin. samplewere then processed through different stages of dehydration, clearing and impregnation and finally embedded in paraffin blocks. sections of 5dm thickness then cut and stained by alkaline congo red techniques. spleen weights and the data obtained from microscopic quantitative analysis did show no significant differences between groups a and b, a andc, and b and c. but significant differences were observed between groups a and d, b and d, and c and drespectively. itis concluded that two compounds ie; agno3 and freund's adjuvant have the same potential, as doesvitamin free casein have, in spatio - temporal pattem of experimental amyloidosis in mice