تاثیر دوازده هفته تمرینات ترکیبی بر تنظیم نشانگرهای التهابی زنان یائسۀ مستعد سرطان سینه

مقدمه: هدف از پژوهش حاضر تعیین اثر تمرین ترکیبی بر بیان ژن برخی از سایتوکاین‌های پیش‌التهابی و ارزیابی سطوح هورمونی در زنان یائسۀ چاق است.روش پژوهش: در این تحقیق نیمه‌تجربی 30 زن یائسۀ کم‌تحرک و چاق انتخاب شده و به‌طور تصادفی به دو گروه تجربی (15 نفر) و گروه کنترل (15 نفر) تقسیم شدند. گروه تجربی به مدت 12 هفته، پنج روز در هفته، شامل دو جلسه تمرین مقاومتی به مدت 60 دقیقه و 3 جلسه تمرین هوازی به مدت 30 دقیقه، شرکت کردند. پیش و پس از مداخله، نمونۀ خون برای ارزیابی استرادیول، استرادیول آزاد و میزان بیان mrna ژن‌های اینترلوکین-6 (il-6) و تومور نکروزدهندۀ آلفا (tnf-α) از داوطلبان اخذ شد. بیان کمی ژن با استفاده از روش 2-∆∆ct و از طریق نرم‌افزار rest ارزیابی شد. از آزمون شاپیروویلک برای بررسی توزیع طبیعی داده‌ها و از تحلیل واریانس در اندازه‌گیری‌های مکرر برای مقایسة گروه‌ها در سطح معناداری α<0.05  استفاده شد.یافته‌ها: در پاسخ به پروتکل تمرینی، کاهش در‌ تودۀ چربی (9.6- درصد) و افزایش تودۀ بدون چربی (4.2+ درصد) در گروه تجربی مشاهده شد. سطوح استرادیول آزاد (70.10- درصد ) و میزان بیان ژن il-6 ( p=0.003) و tnf-α (p=0.000) کاهش معناداری در گروه تجربی نسبت به گروه کنترل داشت. اما تفاوت معناداری در استرادیول برای گروه تجربی وجود نداشت (p<0.05).نتیجه‌گیری: به‌نظر می‌رسد تمرین ترکیبی و کاهش تودۀ چربی ناشی از فعالیت، پروفایل هورمون‌های جنسی و میزان بیان ژن il-6 و tnf-α را در زنان یائسۀ مستعد bc تغییر می‌دهد. بنابراین این تحقیق شواهد بیشتری از تعامل پیچیدۀ بین نشانگرهای التهابی، بافت چربی و تودۀ عضلانی را در این گروه از زنان ارائه می‌کند.

the effect of twelve-week concurrent training on the regulation of inflammatory markers in postmenopausal women prone to breast cancer

introduction: the present study aimed to determine the effect of 12-week concurrent training on gene expression of some pro-inflammatory cytokines and to evaluate hormone levels of obese postmenopausal women.methods: in this quasi-experimental research, 30 sedentary and obese pw were recruited and randomly divided into concurrent training (ct, n=15) and control (cg, n=15) groups. participants of the experimental group took part in 12-week supervised intervention training for five days a week, including two sessions of 60-minute resistance training and three sessions of 30-minute aerobic training. blood samples were taken from the volunteers to measure estradiol, free estradiol, and mrna expression levels of interleukin-6 (il-6) and tumor necrosis factor-alpha (tnf-α). quantitative gene expression was evaluated using the 2-∆∆ct method and rest software. the shapiro-wilk test was used to check the normal distribution of the data, and repeated measurements analysis of variance (anova) tests were used to compare the groups at a significance level of α=0.05.results: in response to the training protocol, the ct group experienced a reduction in fat mass (-9.6%; p<0.05) and an increase in lean body mass (2.4%; p<0.05). the circulating levels of free estradiol (−10.70%; p<0.05), and levels of gene expression of il-6 (p=0.03) and tnf-α (p=0.000) significantly decreased in the ct group compared with the cg group. but there was no significant difference in estradiol in the ct group compared with the cg group (p<0.05).conclusion: the concurrent training and exercise-induced fat mass loss seem to modify the sex hormones profile and levels of gene expression of il-6 and tnf-α in ‌pw prone to breast cancer. thus, this study provides ‌additional evidence of the intricate interaction of inflammatory markers, adipose tissue, and muscle ‌mass in pw prone to breast cancer.‌introduction: the present study aimed to determine the effect of 12-week concurrent training on gene expression of some pro-inflammatory cytokines and to evaluate hormone levels of obese postmenopausal women.methods: in this quasi-experimental research, 30 sedentary and obese pw were recruited and randomly divided into concurrent training (ct, n=15) and control (cg, n=15) groups. participants of the experimental group took part in 12-week supervised intervention training for five days a week, including two sessions of 60-minute resistance training and three sessions of 30-minute aerobic training. blood samples were taken from the volunteers to measure estradiol, free estradiol, and mrna expression levels of interleukin-6 (il-6) and tumor necrosis factor-alpha (tnf-α). quantitative gene expression was evaluated using the 2-∆∆ct method and rest software. the shapiro-wilk test was used to check the normal distribution of the data, and repeated measurements analysis of variance (anova) tests were used to compare the groups at a significance level of α=0.05.results: in response to the training protocol, the ct group experienced a reduction in fat mass (-9.6%; p<0.05) and an increase in lean body mass (2.4%; p<0.05). the circulating levels of free estradiol (−10.70%; p<0.05), and levels of gene expression of il-6 (p=0.03) and tnf-α (p=0.000) significantly decreased in the ct group compared with the cg group. but there was no significant difference in estradiol in the ct group compared with the cg group (p<0.05).conclusion: the concurrent training and exercise-induced fat mass loss seem to modify the sex hormones profile and levels of gene expression of il-6 and tnf-α in ‌pw prone to breast cancer. thus, this study provides ‌additional evidence of the intricate interaction of inflammatory markers, adipose tissue, and muscle ‌mass in pw prone to breast cancer.‌introduction: the present study aimed to determine the effect of 12-week concurrent training on gene expression of some pro-inflammatory cytokines and to evaluate hormone levels of obese postmenopausal women.methods: in this quasi-experimental research, 30 sedentary and obese pw were recruited and randomly divided into concurrent training (ct, n=15) and control (cg, n=15) groups. participants of the experimental group took part in 12-week supervised intervention training for five days a week, including two sessions of 60-minute resistance training and three sessions of 30-minute aerobic training. blood samples were taken from the volunteers to measure estradiol, free estradiol, and mrna expression levels of interleukin-6 (il-6) and tumor necrosis factor-alpha (tnf-α). quantitative gene expression was evaluated using the 2-∆∆ct method and rest software. the shapiro-wilk test was used to check the normal distribution of the data, and repeated measurements analysis of variance (anova) tests were used to compare the groups at a significance level of α=0.05.results: in response to the training protocol, the ct group experienced a reduction in fat mass (-9.6%; p<0.05) and an increase in lean body mass (2.4%; p<0.05). the circulating levels of free estradiol (−10.70%; p<0.05), and levels of gene expression of il-6 (p=0.03) and tnf-α (p=0.000) significantly decreased in the ct group compared with the cg group. but there was no significant difference in estradiol in the ct group compared with the cg group (p<0.05).conclusion: the concurrent training and exercise-induced fat mass loss seem to modify the sex hormones profile and levels of gene expression of il-6 and tnf-α in ‌pw prone to breast cancer. thus, this study provides ‌additional evidence of the intricate interaction of inflammatory markers, adipose tissue, and muscle ‌mass in pw prone to breast cancer.‌