اثرات تمرین هوازی بر شاخص‌های استرس اکسایشی کلیه و مقدار nox4 ﺳﺮم ﻣﻮشﻫﺎی ﺻﺤﺮاﯾﯽ ﻣﺪل ﺳﮑﺘﮥ ﻗﻠﺒﯽ

در پی سکتۀ قلبی (mi) آسیب‌‌های کلیوی ناشی از استرس اکسایشی شدت می‌گیرند که با تغییر بیان nox4 همراه است. از ﺳﻮﯾﯽ، ﺗﻤﺮﯾﻦ ﺑﺪﻧﯽ تاثیرات ﻣﺜﺒﺘﯽ ﺑﺮ ﺳﺎﺧﺘﺎر و ﻋﻤﻠﮑﺮد ﮐﻠﯿﻪ دارد. در اﯾﻦ ﺗﺤﻘﯿﻖ ﺗﺄﺛﯿﺮ ﺗﻤﺮﯾﻦ هوازی ﺑﺮ ﻣﻘﺪار nox4 ﺳﺮم و ﺷﺎﺧﺺﻫﺎی آﺳﯿﺐ اﮐﺴﺎﯾﺸﯽ ﮐﻠﯿﻪ ﻣﺘﻌﺎﻗﺐ mi ﺑﺮرﺳﯽ ﺷﺪ. روش‌ پژوهش: 24 موش صحرایی نر به‌طور‌تصادفی به سه گروه(8=n) شامل سالم‌کنترل، سکته‌کنترل و سکته‌تمرین تقسیم‌ شدند. پس از القاء سکته قلبی توسط تزریق ایزوپروترنول(100 mg/kg.day) در دو روز متوالی، 8 هفته تمرین دویدن (با شیب صفر) با شدت متوسط روی نوار گردان انجام گرفت ﮐﻪ از 10 ﻣﺘﺮ در دﻗﯿﻘﻪ ﺑﺎ ﻣﺪت 15 دﻗﯿﻘﻪ در ﻫﻔﺘﮥ اول، ﺑﻪ 25 ﻣﺘﺮ در دﻗﯿﻘﻪ و ﻣﺪت 30 دﻗﯿﻘﻪ در ﻫﻔﺘﮥ آﺧﺮ رﺳﯿﺪ. ﻣﻘﺪار nox4 ﺳﺮم ﺑﻪ روش ﻓﻠﻮﺳﯿﺘﻮﻣﺘﺮی، ﻣﻘﺪار mda، ﭘﺮوﺗﺌﯿﻦ ﮐﺮﺑﻮﻧﯿﻠﻪ (cp) و ohdg8 ﮐﻠﯿﻪ ﺑﻪ روش ﻃﯿﻒﺳﻨﺠﯽ و رﻧﮓﺳﻨﺠﯽ اﻧﺠﺎم ﮔﺮﻓﺖ و دادهﻫﺎ ﺑﺎ ﺗﺤﻠﯿﻞ وارﯾﺎﻧﺲ ﺗﮑﺮاﻫﻪ ﺗﺤﻠﯿﻞ ﺷﺪﻧﺪ. ﯾﺎﻓﺘﻪﻫﺎ: درﺣﺎﻟﯽﮐﻪ اﻟﻘﺎی mi ﺳﺒﺐ اﻓﺰاﯾﺶ معنادار ﻣﻘﺪار nox4 ﺳﺮم و ﻫﻤﭽﻨﯿﻦ cp ،mda و 8ohdg ﮐﻠﯿﻪ ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﺳﺎﻟﻢ ﮐﻨﺘﺮل ﺷﺪ، اﻣﺎ اﻧﺠﺎم ﺗﻤﺮﯾﻦ در ﮔﺮوه ﺳﮑﺘﻪ ﺗﻤﺮﯾﻦ ﺳﺒﺐ ﮐﻤﺘﺮ ﺷﺪن ﻣﻘﺪار اﯾﻦ ﺷﺎﺧﺺﻫﺎ ﺑﻪﺟﺰ p=0/056) cp) ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﺳﮑﺘﻪ ﮐﻨﺘﺮل ﺷﺪ (0/001=p). ﺑﺎ اﯾﻦ ﺣﺎل، ﻣﻘﺪار ﻫﺮ ﭼﻬﺎر ﺷﺎﺧﺺ ﺷﺎﻣﻞ nox4 ﺳﺮم (0/029=p) و ﻫﻤﭽﻨﯿﻦ p=0/001) cp ،)p=0/001) mda) و p=0/019) 8ohdg) ﮐﻠﯿﻪ در ﮔﺮوه ﺳﮑﺘﻪ ﺗﻤﺮﯾﻦ (ﺑﺎ وﺟﻮد ﺑﻬﺒﻮد ﭼﺸﻤﮕﯿﺮ)، ﻫﻨﻮز ﻫﻢ ﺑﯿﺸﺘﺮ از ﮔﺮوه ﺳﺎﻟﻢ ﮐﻨﺘﺮل ﺑﻮد. ﻧﺘﯿﺠﻪﮔﯿﺮی: mi ﻣﯽﺗﻮاﻧﺪ ﺳﺒﺐ اﻓﺰاﯾﺶ nox4 ﺳﺮم و ﺑﺮوز اﺳﺘﺮس اﮐﺴﺎﯾﺸﯽ در ﮐﻠﯿﻪ ﺷﻮد. ﺑﺎ اﯾﻦ ﺣﺎل، ﺗﻤﺮﯾﻦ ﻫﻮازی ﺑﺎ وﺟﻮد ﻣﻌﮑﻮس ﮐﺮدن ﻗﺎﺑﻞ ﻣﻼﺣﻈﮥ اﯾﻦ روﻧﺪ، ﻗﺎدر ﺑﻪ رﻓﻊ ﮐﺎﻣﻞ اﯾﻦ ﺗﺄﺛﯿﺮات mi ﻧﺒﻮد. ﺑﻪﻧﻈﺮ ﻣﯽرﺳﺪ ﮐﻪ در ﺗﺤﻘﯿﻘﺎت آﯾﻨﺪه ﻋﻼوهﺑﺮ رﻓﻊ ﻣﺤﺪودﯾﺖﻫﺎی ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ، ﻧﯿﺎز ﺑﻪ ﺷﻨﺎﺳﺎﯾﯽ ﺑﻬﺘﺮﯾﻦ ﭘﺮوﺗﮑﻞﻫﺎی ﺗﻤﺮﯾﻨﯽ ﻣﺆﺛﺮ در اﯾﻦ زﻣﯿﻨﻪ ﺑﺎﻗﯽ اﺳﺖ.

effects of aerobic training on markers of renal oxidative stress and serum nox4 level in rats with myocardial infarction model

oxidative stress induced renal injures are intensified following myocardial infraction(mi) which are accompanied by an elevated nox4 expression rate. however; the effect of exercise training following mi on blood nox4 and renal oxidative stress markers have not investigated in spite of the apparent evidence regarding the effects of physical training on renal structure and function, which was investigated in this study. 24 male wistar rats were randomized into three groups (n=8) of healthy control, mi control and mi training. eight weeks of running training at moderate intensity on a treadmill was conducted following mi induction via two executive days’ injections of isoproterenol (100 mg/kg.day). while renal mda, cabonylated protein(cp) and 8ohdg levels via spectrophotometry and colorimeterywhile blood nox4 as well as renal mda, cp and 8ohdg levels in mi control rats were higher than their healthy control littermates, the amount of these parameters (except for cp) in mi group was lower than healthy control group (p=0.001 in any circumstances). however, all indices including on blood nox4 (p=0.029) and renal mda(p=0.001), cp(p=0.001) and 8ohdg(p=0.019) levels in mi training rats was still higher than healthy control littermates. mi is able to increase blood nox4 level and incidence of renal oxidative stress. however, aerobic training could not fully ameliorate these hazardous effects of mi, in spite of its appreciable capabilities to reverse this trend. it seems that identification of the best effective training protocols in this area is warranted in addition to eliminating the limitations in this study.