Recyclable of Lipozyme RM IM in biodiesel synthesis from CPO through interesterification process

Synthesis of biodiesel using immobilized lipase is promising because of its ability to fix the disadvantages of using an alkali catalyst such as non-occurrence of side reactions (formation of soap) and separation of the catalyst from product more easily,and most significant advantage is that it could be recycle. usually,enzymatic biodiesel production has been studied by previous researchers using refined oil as feedstock. cost of biodiesel produced from refined oil is higher than fossil fuel. recycled lipozyme rm im to produced biodiesel from crude palm oil (cpo) as feedstock has been investigated. degumming process for crude palm oil as a pretreatment has done to removed impurities in form of gum and phosphatides compound degumming process was also decreased content of ffa reaches 14.33%. degumming process has to be done because gum and phosphatides compounds strongly adsorbed on the surface of lipozyme rm im. in this research,synthesis of biodiesel from cpo through interesterification (using of methyl acetate as acyl acceptor) and recycled lipozyme rm im as catalysts in a batch reactor to obtain biodiesel as product were analyzed using gas chromatography (gc). as a study in this research,discussed the decreasing of lipase activity while recycled that seen from the acquisition yield of biodiesel as a result. the best result showed that decreasing lipase activity obtained in this research was 16.76% after 3 times with the conditions of 1:6 molar ratio,temperature of reaction 45 ° c and amount of lipozyme rm im was 26%. the effect of molar ratio and amount of lipozyme rm im were investigated as the cause of decrease in enzyme activity after recycled. © school of engineering,taylor’s university.