Numerical solution of steady state dispersion flow model for lactose-lactase hydrolysis with different kinetics in fixed bed

A detailed computational procedure for evaluating lactose hydrolysis with immobilized enzyme in a packed bed tubular reactor under dispersion flow conditions is presented. the dispersion flow model for lactose hydrolysis using different kinetics,taking cognizance of external mass transfer resistances,was solved by the method of orthogonal collocation. the reliability of model simulations was tested using experimental data from a laboratory packed bed column,where the β-galactosidase of kluyveromyces fragilis was immobilized on spherical chitosan beads. comparison of the simulated results with experimental exit conversion shows that the dispersion flow model and using michaelis-menten kinetics with competitive product (galactose) inhibition are appropriate to interpret the experimental results and simulate the process of lactose hydrolysis in a fixed bed. © school of engineering,taylor's university college.