Fabrication of Bovine Serum Albumin Nanoparticles Self-assembled Coacervation Method for Drug Delivery Systems

Bovine serum albumin (bsa) rianoparticles have been produced in the particle size between 112 to468 (om). nanoparticles were purified with 48,800 g centrifuge following by dialysis, micro and ultra filtration.photon correlation spectroscopy (pcs) and scanning electron microscopy (sem) were used to characterizethe nanoparticles, photographs obtained from sem image shown nanoparticles have semi-spherical shape.however the influence of several parameters such as the rate of addition desolvating agent,the concentrationof bsa, the gluttaraldehyde concentration, agitation speed and bsajethanol volumetric ratio have beeninvestigated while the temperature kept in 14°c.the mean nanoparticle size diameter has not been relativelychanged by variation of solvent adding rate and cross linker concentration and in contrast mainly dependedon the volumetric ratio of bsa! ethanol, bsa concentration and agitation speed.