determination of ethyl carbamate in sugar cane spirit by direct injection electrospray ionization tandem mass spectrometry using 18-crown-6/trifluoroacetic acid spiking additives

In this work, we describe a 2-min direct injection electrospray tandem quadrupolar mass spectrometric method for ethyl carbamate (ec) detection in brazilian sugar cane spirit (scs). analyses were carried out with 18-crown-6/trifluoracetic acid (30 ppm/0.1%) additives to sequester metal cations by eliminating unwanted [ec + na]+ and [ec + k]+ ions, favoring analyte detection as sole [ec + h]+ ions. standard addition of ec was carried out with 18-crown-6/trifluoracetic acid (30 ppm/0.1%), resulting in spiked scs samples (0, 75, 150, 300, 450, 600, 750, and 900 μg l−1). a carrier solution of methanol:18-crown-6:trifluoroacetic acid (99.9:30 ppm:0.10, v/v/v) was pumped to the mass spectrometer using positive ion mode and multiple reaction monitoring of 90 > 62 for ec. the limits of detection and quantification were 7.5 and 75.0 μg l−1, respectively, and the dynamic range used was 75–900 μg l−1. the validation results and application of the method to the analysis of real samples and certified scs demonstrate its applicability to quality control and regulatory analysis.