use of functionalized covalent organic framework as sorbent for the solid-phase extraction of biogenic amines from meat samples followed by high-performance liquid chromatography

A functionalized covalent organic framework (cof) was synthesized via room-temperature solvent-free mechano-chemical grinding method and used as a solid-phase extraction adsorbent for the extraction of eight biogenic amines (tryptamine, n-butylamine, phenylethylamine, cadaverine, putrescine, tyramine, spermine, and spermidine) in diverse meat samples. the target biogenic amines were derived with dansyl chloride to form stable fluorogenic derivatives for their high-performance liquid chromatography–fluorescence detection. the main important experimental parameters were investigated and optimized by both one-factor-at-a-time and response surface methodologies. under the optimum conditions, a linear response for the biogenic amines was observed in the concentration range of 5.0–800.0 μg l−1 with the coefficients of determination (r2) ranging from 0.9944 to 0.9981. the limits of detection of this method (at a signal-to-noise ratio of 3) for the analytes ranged from 0.92 to 2.57 μg l−1. the method recoveries of the target biogenic amines for five meat samples ranged from 80.3 to 115%, with relative standard deviations lower than 12%. the results indicate that the prepared cof is an effective adsorbent for the enrichment of biogenic amines.