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dual-mode aptasensor for sers and chiral detection of campylobacter jejuni
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نویسنده
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he deyun ,wu zhengzong ,cui bo ,xu enbo
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منبع
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food analytical methods - 2019 - دوره : 12 - شماره : 10 - صفحه:2185 -2193
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چکیده
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Campylobacter jejuni (c. jejuni), a foodborne pathogen, is a major contributor to human bacterial gastroenteritis worldwide and detrimental to public health. in this paper, we developed a dual-mode aptasensor for campylobacter jejuni (c. jejuni) determination. based on surface-enhanced raman scattering (sers) and chiral optical response, the concentrations of c. jejuni in food products can be simultaneously quantified by sers and chirality methods. under the optimized conditions, in the ranges of 102–5 × 106 cfu/ml and 102–106 cfu/ml, the concentration of c. jejuni exhibited a good linear relationship (r2 = 0.9905 and 0.9819) with changed sers signal intensity and chiral signal intensity, respectively. in addition, the applicability of the novel aptasensor was validated by detecting different concentrations of c. jejuni spiked in milk samples. with its satisfactory performances, this bimodal aptasensor is conceivably to be a promising candidate for determining c. jejuni in food products.
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کلیدواژه
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dual-mode ,surface-enhanced raman scattering ,circular dichroism ,foodborne pathogen ,campylobacter jejuni
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آدرس
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qilu university of technology, college of food science and engineering, shandong academy of sciences, state key laboratory of biobased material and green papermaking, china. china agricultural university, college of biological sciences, state key laboratory of plant physiology and biochemistry, china, qilu university of technology, college of food science and engineering, shandong academy of sciences, state key laboratory of biobased material and green papermaking, china, qilu university of technology, college of food science and engineering, shandong academy of sciences, state key laboratory of biobased material and green papermaking, china, zhejiang university, college of biosystems engineering and food science, fuli institute of food science, national engineering laboratory of intelligent food technology and equipment, zhejiang key laboratory for agro-food processing, china
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Authors
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