|
|
|
|
colorimetric immunoassay for the detection of staphylococcus aureus by using magnetic carbon dots and sliver nanoclusters as o-phenylenediamine-oxidase mimetics
|
|
|
|
|
|
|
|
نویسنده
|
yao shuo ,zhao chao ,liu yushen ,nie heran ,xi gaolei ,cao xiaolian ,li zhuolin ,pang bo ,li juan ,wang juan
|
|
منبع
|
food analytical methods - 2020 - دوره : 13 - شماره : 4 - صفحه:833 -838
|
|
چکیده
|
Staphylococcus aureus (s. aureus) is an important foodborne pathogen that causes food poisoning and severe infections. herein, we design a novel and sensitive colorimetric immunoassay for the detection of s. aureus based on the formation of a magnetic bead-based sandwich complex and signal amplification via an enzyme mimic. magnetic nanoparticles decorated with carbon dots offer rapid enrichment and separation of target bacteria in complex matrices. sliver nanoclusters are used as an artificial enzyme that can oxidize o-phenylenediamine to form a yellow product with a maximum absorption peak at 421 nm. this method can visually detect s. aureus ranging from 10 to 106 cfu ml−1. its limit of detection reaches down to 4.9 cfu ml−1. by analyzing food samples, the recovery is from 85.6 to 103.7%.
|
|
کلیدواژه
|
food safety ,staphylococcus aureus ,visual detection ,immunomagnetic separation ,nanozyme ,o-phenylenediamine
|
|
آدرس
|
jilin university, school of public health, people’s republic of china, jilin university, school of public health, people’s republic of china, jilin university, school of public health, people’s republic of china, chinese academy of sciences, changchun institute of applied chemistry, key laboratory of polymer ecomaterials, people’s republic of china, technology center for china tobacco henan industrial limited company, people’s republic of china, jilin university, school of public health, people’s republic of china, jilin university, school of public health, people’s republic of china, jilin university, school of public health, people’s republic of china, jilin university, school of public health, people’s republic of china, jilin university, school of public health, people’s republic of china
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|