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alkaline phosphatase–triggered immunoassay based on fluorogenic reaction for sensitive detection of acetochlor, metolachlor, and propisochlor
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نویسنده
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zha yonghong ,liu xiaolei ,hu pan ,lu shiying ,ren honglin ,liu zengshan ,liang xiongyan ,yang yuying ,li yansong ,zhou yu
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منبع
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food analytical methods - 2020 - دوره : 13 - شماره : 4 - صفحه:1008 -1016
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چکیده
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Enzyme-linked immunosorbent assay (elisa), as an immunoassay platform, usually suffers from poor sensitivity due to the limited signal intensity. to overcome this obstacle, an alkaline phosphatase–triggered immunoassay based on fluorogenic reaction of o-phenylenediamine and ascorbic acid was developed for sensitive detection of chloroacetamide herbicides in corn. the new immunoassay revealed limits of detection (lods) for acetochlor (atc), metolachlor, and propisochlor of 1.19 ng/ml, 2.34 ng/ml, and 3.40 ng/ml, respectively. the average recoveries of the three chloroacetamide herbicides in corn sample were 88.4%, 91.8%, and 87.6%, respectively. such fluorescent immunoassay via in situ formation of fluorescent signal from scratch provides a new strategy with excellent sensitivity in point-of-care test of chloroacetamide herbicides.
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کلیدواژه
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fluorescent immunoassay ,chloroacetamide herbicide ,fluorogenic reaction
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آدرس
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ministry of education, jilin university, institute of zoonosis, college of veterinary medicine, key laboratory of zoonoses research, people’s republic of china, ministry of education, jilin university, institute of zoonosis, college of veterinary medicine, key laboratory of zoonoses research, people’s republic of china, ministry of education, jilin university, institute of zoonosis, college of veterinary medicine, key laboratory of zoonoses research, people’s republic of china, ministry of education, jilin university, institute of zoonosis, college of veterinary medicine, key laboratory of zoonoses research, people’s republic of china, ministry of education, jilin university, institute of zoonosis, college of veterinary medicine, key laboratory of zoonoses research, people’s republic of china, ministry of education, jilin university, institute of zoonosis, college of veterinary medicine, key laboratory of zoonoses research, people’s republic of china, yangtze university, college of animal sciences, people’s republic of china, yangtze university, college of animal sciences, people’s republic of china, ministry of education, jilin university, institute of zoonosis, college of veterinary medicine, key laboratory of zoonoses research, people’s republic of china, ministry of education, jilin university, institute of zoonosis, college of veterinary medicine, key laboratory of zoonoses research, people’s republic of china. yangtze university, college of animal sciences, people’s republic of china
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Authors
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