development and validation of an analytical method for the determination of aloin using liquid chromatography tandem mass spectrometry

A new simple analytical method was developed for the identification and quantification of aloin in aloe gel. the extraction was performed using acetonitrile acidified with formic acid (1%, v/v) with no additional cleanup step. the compound was determined using high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (qqq). the recovery rates obtained were between 80 and 110% with a relative standard deviation < 26%, at four different concentration levels. the calculated limit of quantification (loq) was at 0.05 mg kg−1.