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development of sensitive, rapid, and effective immunoassays for the detection of vitamin b12 in fortified food and nutritional supplements
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نویسنده
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kong dezhao ,liu liqiang ,song shanshan ,kuang hua ,xu chuanlai
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منبع
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food analytical methods - 2017 - دوره : 10 - شماره : 1 - صفحه:10 -18
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چکیده
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An indirect competitive enzyme-linked immunosorbent assay (ic-elisa) method and lateral-flow immunochromatographic (ica) strip assay method were developed for the detection of vitamin b12 (four major forms, cyanocobalamin, hydroxocobalamin, adenosylcobalamin, and methylcobalamin) in different food products. the limit of detection and 50 % inhibitory concentration for the ic-elisa method were 0.065 and 0.43 ng/ml, respectively. the visual limit of detection and cutoff value for the lateral-flow ica strip assay method were 1 and 4 ng/ml, respectively. for the detection of fortified food and nutritional supplements in vitamin tablets, energy drink, and infant milk powder samples, the recovery rates were in the range of 81 to 122 % for the ic-elisa method, and even the lowest content in infant milk powder was identified by the lateral-flow ica strip assay. therefore, both of these methods are sensitive, rapid, and effective and are suitable for the on-site detection and rapid screening of mass samples.
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کلیدواژه
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vitamin b12 ,ic-elisa ,ica ,fortified food and nutritional supplements
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آدرس
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jiangnan university, school of food science and technology, state key laboratory of food science and technology, people’s republic of china, jiangnan university, school of food science and technology, state key laboratory of food science and technology, people’s republic of china, jiangnan university, school of food science and technology, state key laboratory of food science and technology, people’s republic of china, jiangnan university, school of food science and technology, state key laboratory of food science and technology, people’s republic of china, jiangnan university, school of food science and technology, state key laboratory of food science and technology, people’s republic of china
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Authors
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