method development for sulfonylurea herbicides analysis in rapeseed oil samples by hplc–dad: comparison of zirconium-based sorbents and emr-lipid for clean-up of quechers extract

This study develops a simple, cost-effective and sensitive high-performance liquid chromatography with diode array detector (hplc–dad) method for the simultaneous determination of eight sulfonylurea herbicides (oxasulfuron, metsulfuron-methyl, triasulfuron, chlorsulfuron, amidosulfuron, mesosulfuron-methyl, bensulfuron-methyl and tritosulfuron) in rapeseed oil. extraction of target analytes was performed using quick, easy, cheap, effective, rugged and safe-based procedure followed by solid-phase extraction (spe) and dispersive solid-phase extraction (d-spe) clean-up, and presents good performance for all of the analytes with recoveries in the range of 67–133% and relative standard deviations (rsd) less than 15%. no significant matrix interference was observed due to the application of effective zirconium dioxide-based sorbent (z-sep). method loqs for most of the investigated analytes were set at satisfactory low value of 20 ng g−1 in food product. the procedure was evaluated in analyses of actual samples. the most important steps of the method optimization are presented. novel emr-lipid clean-up solution for samples with high fat content was evaluated and compared to z-sep sorbent.