application of high-performance liquid chromatography with diode array detector for simultaneous determination of 11 synthetic dyes in selected beverages and foodstuffs

A simple, inexpensive and robust high-performance liquid chromatography diode array detector (hpc-dad) procedures are proposed to analyse food dyes in beverages, hard candy and fish roe samples. an ether-linked phenyl stationary phase provides sufficient selectivity and chromatographic performance for separation of 11 sulfonated azo dyes. beverage samples were only diluted (and degassed when needed) before analysis. solid-phase extraction (spe) or matrix solid-phase dispersion (mspd) procedures are proposed for efficient extraction of the analytes from candies or fish roe samples, respectively. limits of detection (lods) were from 0.005 to 0.013 μg ml−1 and limits of quantification (loqs) between 0.014 and 0.038 μg ml−1. hplc-dad method was validated in terms of intra- and inter-day accuracy and precision at three concentration levels 2, 1, and 0.1 μg ml−1. validation was also performed for spe and mspd extraction procedures including intra- and inter-day accuracy (recovery %) and precision (rsd%), as well as intra-laboratory reproducibility. application to analysis of beverages and food samples available to consumers proved that described methods are suitable for the routine analysis of dyes in food products.