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development of a nanobody-based competitive dot elisa for visual screening of ochratoxin a in cereals
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نویسنده
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sun zhichang ,duan zhenhua ,liu xing ,deng xing ,tang zongwen
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منبع
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food analytical methods - 2017 - دوره : 10 - شماره : 11 - صفحه:3558 -3564
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چکیده
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A polyvinylidene fluoride membrane-based dot immunoassay using nanobody (nb) for rapid, qualitative, and visual detection of ochratoxin a (ota) in cereals was developed. on the basis of optimal assay conditions, the cut-off level of this method assessed visually was 5 μg/kg for ota, and the final results were obtained within 20 min. this method was simple with no time-consuming cleanup procedure. good accuracy and reproducibility were obtained in recovery experiments. results of the nb-based dot elisa are in agreement with the elisa kit, except for samples that were negative for ota presence assessed through nb-based dot elisa but found positive through the elisa kit. these results indicated that the developed method could be a useful on-site screening tool for rapid detection of ota in cereals without special instrument.
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کلیدواژه
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ochratoxin a ,nanobody ,cereal ,dot elisa ,polyvinylidene fluoride membrane
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آدرس
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hezhou university, institute of food research, china. hainan university, college of food science and technology, china, hezhou university, institute of food research, china, hezhou university, institute of food research, china. hainan university, college of food science and technology, china, hainan university, college of food science and technology, china, hainan university, college of food science and technology, china
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Authors
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