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quantification of piperazine in chicken muscle by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry
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نویسنده
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xie kaizhou ,liu ya’nan ,sun lirui ,pang maoda ,xie xing ,gao qiang ,wang bo ,zhang yangyang ,wang ran ,zhang genxi ,dai guojun ,wang jinyu
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منبع
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food analytical methods - 2017 - دوره : 10 - شماره : 6 - صفحه:1736 -1744
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چکیده
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An ultra-performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry (uplc-esi/ms/ms) method was developed for the detection of piperazine in chicken muscle. following extraction and purification, the chicken muscle extract was injected into the uplc system and analyzed. piperazine detection was performed on a triple-quadrupole mass spectrometer with the esi interface operating in positive mode. the most sensitive mass transition from the precursor ion to the product ion was 87.1 → 44.1 for piperazine. the coefficient (r 2) of piperazine was 0.9995 in the range of 1–200 μg/kg. the recovery and relative standard deviation (rsd) ranged from 102.93 to 111.46% and from 4.57 to 5.28% at 50.0, 100.0 and 200.0 μg/kg adding levels, respectively. limits of detection (lods) and limits of quantification (loqs) were 0.4 and 1.0 μg/kg, respectively. this method was fully validated based on its specificity, sensitivity, linearity, precision, accuracy, matrix effect, and stability results. compared to other researches, this new method not only omitted the derivative process, which simplify the pre-processing, but also proved a better sensitivity based on its low lod and loq values.
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کلیدواژه
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piperazine ,uplc-esi/ms/ms ,chicken muscle
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آدرس
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yangzhou university, college of animal science and technology, joint international research laboratory of agriculture and agri-product safety, china. key laboratory for animal genetic, china, yangzhou university, college of animal science and technology, joint international research laboratory of agriculture and agri-product safety, china. key laboratory for animal genetic, china, yangzhou university, college of animal science and technology, joint international research laboratory of agriculture and agri-product safety, china. key laboratory for animal genetic, china, jiangsu academy of agricultural sciences, china, jiangsu academy of agricultural sciences, china, yangzhou university, college of animal science and technology, joint international research laboratory of agriculture and agri-product safety, china. key laboratory for animal genetic, china, yangzhou university, college of animal science and technology, joint international research laboratory of agriculture and agri-product safety, china. key laboratory for animal genetic, china, yangzhou university, college of animal science and technology, joint international research laboratory of agriculture and agri-product safety, china. key laboratory for animal genetic, china, jiangsu academy of agricultural sciences, china, yangzhou university, college of animal science and technology, joint international research laboratory of agriculture and agri-product safety, china. key laboratory for animal genetic, china, yangzhou university, college of animal science and technology, joint international research laboratory of agriculture and agri-product safety, china. key laboratory for animal genetic, china, yangzhou university, college of animal science and technology, joint international research laboratory of agriculture and agri-product safety, china. key laboratory for animal genetic, china
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Authors
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