fatty acids analysis using gas chromatography-mass spectrometer detector (gc/msd) - method validation based on berry seed extract samples

Oily seed extracts were used as research samples in analytical laboratory of raw materials and plant products. supercritical carbon dioxide extraction on an industrial scale was used to prepare extracts from raw plant materials, usually berry seeds: blackcurrant, raspberry, strawberry, and chokeberry. gas chromatography analysis was performed on agilent equipment with single quadrupole mass spectrometer detector and split/splitless injector. fatty acids (fas) were determined using indirect method, where they were converted to corresponding methyl esters (fames) during trimethylsulfonium hydroxide solution reaction. comparing the mass spectrum and retention time peak, more than 30 compounds in different oily extracts were qualitatively identified. quantification of individual fatty acids was based on two different methods. firstly, it was based on the received peak area, and the results were normalized without correction factor. secondly, the quantification was based on external calibration curve, for 34 identified fatty acids. the method was validated, and the results, e.g., linearity, precision, limit of detection (lod), and limit of quantification (loq), were presented.