comprehensive method validation for the determination of 170 pesticide residues in pear employing modified quechers without clean-up and ultra-high performance liquid chromatography coupled to tandem mass spectrometry

A fast ultra-high performance liquid chromatography coupled to tandem mass spectrometry (uhplc-ms/ms) method was developed for quantitative determination of pesticide residues in pear. a fast modified acetate quechers method without clean-up was used for sample preparation. validation was performed according to sante guideline. matrix effect results were significative for most part of compounds and thus a matrix-matched calibration was employed. the linear range of the method was from 2.5 to 100 μg kg−1. recoveries were between 70 and 120% with precision ≤ 20%. limit of quantification was 2.5 μg kg−1 for most compounds. uncertainty results ranged from 22 to 49%. in real sample analyses, 21 compounds were quantified in concentrations between 3.3 and 1427 μg kg−1. method proved to be simple, robust and effective to be applied in routine analysis.