characterization of separation and purification technology and identification of taurine from the bovine liver

The present study reports an effective method using cation exchange adsorption during the separation and purification of taurine from bovine liver. we use cation exchange method, crystallization and recrystallization, tlc analysis, and infrared spectrometry. the results indicated that the optimum parameters of cation exchange preliminarily separation and purification of taurine are as follows: a 5.66-ml injection volume, a ph value of 4.94, and an elution rate of 2.5 ml/min. taurine pure product was obtained by crystallization and recrystallization method, its purity more than 98.1%. the results of infrared spectroscopy showed that the purified taurine contain the absorption peak of –nh2 (3212.83, 3045.05, 2971.77 cm−1) and the absorption peak of –so3h (1184.08, 1305.57, 1511.92, 1585.20, 1616.06 cm−1), consistent with the infrared absorption peaks of taurine standards. the method of separation and purification out of taurine from bovine liver is successful and effectively improves the purity of taurine crude products; it can be applied to industrial production.